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Mineralocorticoid receptor (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>) activation plays an important role in the pathogenesis of multiple cardiovascular diseases. Here, we examined whether <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> silencing in bone marrow derived mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) could improve <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s\u2019 survival and enhance their cardioprotective effects in <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s from male Sprague\u2010Dawley rats were transfected with adenoviral small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> to silence <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> silencing decreased hypoxia\u2010induced <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s\u2019 apoptosis, as demonstrated by Annexin V\/7\u2010<jats:styled-content style=\"fixed-case\">AAD<\/jats:styled-content> staining. The mechanisms contributing to the beneficial effects of <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> depletion were associated with inhibiting intracellular reactive oxygen species production and increased Bcl\u20102\/Bax ratio. In vivo study, 1\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> of <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s with or without si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> were injected into rat hearts immediately after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>. Depletion of <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> could improve the <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s\u2019 survival significantly in infarcted myocardium, associated with more cardiac function improvement and smaller infarct size. Capillary density were also significantly higher in si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> group with increased expression of vascular endothelial growth factor. Our study demonstrated that silencing <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> promoted <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s\u2019 survival and repair efficacy in ischaemic hearts. <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> might be a potential target for enhancing the efficacy of cell therapy in ischaemic heart disease.<\/jats:p>","DOI":"10.1111\/jcmm.14026","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T05:10:24Z","timestamp":1544764224000},"page":"1246-1256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mineralocorticoid receptor deficiency improves the therapeutic effects of mesenchymal stem cells for myocardial infarction via enhanced cell survival"],"prefix":"10.1111","volume":"23","author":[{"given":"Xinxing","family":"Xie","sequence":"first","affiliation":[{"name":"Department of 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