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In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo\u2010cytoplasmic trafficking of <jats:italic>Scn5a <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. Overexpression of Nup107 induced the protein expression of <jats:italic>Scn5a<\/jats:italic> rather than that of other ion channels, with no effects of their <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels. The analysis for the protein production demonstrated Nup107\u2010facilitated transport of <jats:italic>Scn5a <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. Using <jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and luciferase assay, we found that the 5\u2032\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> of <jats:italic>Scn5a <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> was not involved in the interaction, whereas the spatial interaction between Nup107 protein and <jats:italic>Scn5a <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> was formed when <jats:italic>Scn5a <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> passing through the nuclear pore. Functionally, Nup107 overexpression in neonatal rat ventricle myocytes significantly increased the currents of <jats:italic>Scn5a<\/jats:italic>\u2010encoded I<jats:sub>Na<\/jats:sub> channel. Moreover, the close correlation between Nup107 and Nav1.5 protein expression was observed in cardiomycytes and heart tissues subjected to hypoxia and ischaemic insults, suggesting a fast regulation of Nup107 on Nav1.5 channel in cardiac myocytes in a posttranscriptional manner. These findings may provide insights into the emergent control of cardiac electrophysiology through Nup\u2010mediated modulation of ion channels.<\/jats:p>","DOI":"10.1111\/jcmm.14051","type":"journal-article","created":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T09:50:07Z","timestamp":1543830607000},"page":"1448-1457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nucleoporin 107 facilitates the nuclear export of <i>Scn5a <\/i><scp>mRNA<\/scp> to regulate cardiac bioelectricity"],"prefix":"10.1111","volume":"23","author":[{"given":"Yi","family":"Guan","sequence":"first","affiliation":[{"name":"Heart Health Center East Hospital Tongji University School of Medicine Shanghai China"},{"name":"Institute of Medical Genetics Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Gao","sequence":"additional","affiliation":[{"name":"Heart Health Center 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