{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T12:26:42Z","timestamp":1775737602665,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,25]],"date-time":"2018-12-25T00:00:00Z","timestamp":1545696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31602109"],"award-info":[{"award-number":["31602109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Listeria monocytogenes <\/jats:italic>(LM) is a classical model intracellular pathogen and the leading cause of listeriosis, which has long been a global public health issue. The successful infection of LM is related to a series of virulence factors, such as the transpeptidase enzyme sortase A (SrtA) and listeriolysin O (LLO), which are crucial for bacterial internalization and escape from phagosomes respectively. It is speculated that targeting multiple virulence factors may be due to a synergistic effect on listeriosis therapy. In this study, an active flavonoids component of <jats:italic>Scutellaria baicalensis <\/jats:italic>Georgi, baicalein, was found to potently block both listerial SrtA catalyzed activity and LLO hemolytic activity within 16\u00a0\u03bcg\/mL. After pretreatment with baicalein, 86.30 (\u00b111.35) % of LM failed to associate with Caco\u20102 cells compared to the LM without preincubation (regarded as 100% internalization). Furthermore, baicalein addition may aid in bacterial degradation and clearance in macrophagocytes. During a 5\u00a0h observation, LM in cells incubated with baicalein showed significantly decreased vacuole escapes and sluggish endocellular growth. In addition, baicalein directly prevented LM\u2010induced cells injury and mice fatality (survival rate from 10.00% to 54.55% in 4\u00a0days post\u2010intraperitoneal injection). Taken together, as an antagonist against SrtA and LLO, baicalein can be further developed into a biotherapeutic agent for listeriosis.<\/jats:p>","DOI":"10.1111\/jcmm.14110","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T05:43:40Z","timestamp":1545803020000},"page":"2042-2051","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A potential bio\u2010control agent from baical skullcap root against listeriosis via the inhibition of sortase A and listeriolysin O"],"prefix":"10.1111","volume":"23","author":[{"given":"Gejin","family":"Lu","sequence":"first","affiliation":[{"name":"Center of Infection and Immunity, First Hospital Jilin University  Changchun Jilin, 130021 China"},{"name":"Key Laboratory of Zoonosis, Ministry of Education Institute of Zoonosis, College of Veterinary Medicine, Jilin University  Changchun Jilin, 130062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"Center of Infection and Immunity, First Hospital Jilin University  Changchun Jilin, 130021 China"},{"name":"Key Laboratory of Zoonosis, Ministry of Education Institute of Zoonosis, College of Veterinary Medicine, Jilin University  Changchun Jilin, 130062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Infection and Immunity, First Hospital Jilin University  Changchun Jilin, 130021 China"},{"name":"Key Laboratory of Zoonosis, Ministry of Education Institute of Zoonosis, College of Veterinary Medicine, Jilin University  Changchun Jilin, 130062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6411-1074","authenticated-orcid":false,"given":"Xuming","family":"Deng","sequence":"additional","affiliation":[{"name":"Center of Infection and Immunity, First Hospital Jilin University  Changchun Jilin, 130021 China"},{"name":"Key Laboratory of Zoonosis, Ministry of Education Institute of Zoonosis, College of Veterinary Medicine, Jilin University  Changchun Jilin, 130062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8311-0894","authenticated-orcid":false,"given":"Jianfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Infection and Immunity, First Hospital Jilin University  Changchun Jilin, 130021 China"},{"name":"Key Laboratory of Zoonosis, Ministry of Education Institute of Zoonosis, College of Veterinary Medicine, Jilin University  Changchun Jilin, 130062 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