{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:37:05Z","timestamp":1790638625939,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,25]],"date-time":"2018-12-25T00:00:00Z","timestamp":1545696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005743","name":"Universit\u00e0 Cattolica del Sacro Cuore","doi-asserted-by":"publisher","award":["\u201clinea D1-2017\u201d and \u201clinea D1-2018\u201d"],"award-info":[{"award-number":["\u201clinea D1-2017\u201d and \u201clinea D1-2018\u201d"]}],"id":[{"id":"10.13039\/501100005743","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammation significantly impacts the progression of Huntington's disease (HD) and the mutant HTT protein determines a pro\u2010inflammatory activation of microglia. Mesenchymal stem\/stromal cells (MSC) from the amniotic membrane (hAMSC), and their conditioned medium (CM\u2010hAMSC), have been shown to possess protective effects in vitro and in vivo in animal models of immune\u2010based disorders and of traumatic brain injury, which have been shown to be mediated by their immunomodulatory properties.<\/jats:p><jats:p>In this study, in the R6\/2 mouse model for HD we demonstrate that mice treated with CM\u2010hAMSC display less severe signs of neurological dysfunction than saline\u2010treated ones. CM\u2010hAMSC treatment significantly delayed the development of the hind paw clasping response during tail suspension, reduced deficits in rotarod performance, and decreased locomotor activity in an open field test. The effects of CM\u2010hAMSC on neurological function were reflected in a significant amelioration in brain pathology, including reduction in striatal atrophy and the formation of striatal neuronal intranuclear inclusions. In addition, while no significant increase was found in the expression of BDNF levels after CM\u2010hAMSC treatment, a significant decrease of microglia activation and inducible nitric oxide synthase levels were observed. These results support the concept that CM\u2010hAMSC could act by modulating inflammatory cells, and more specifically microglia.<\/jats:p>","DOI":"10.1111\/jcmm.14113","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T04:07:11Z","timestamp":1545797231000},"page":"1581-1592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Conditioned medium from amniotic cells protects striatal degeneration and ameliorates motor deficits in the R6\/2 mouse model of Huntington's disease"],"prefix":"10.1111","volume":"23","author":[{"given":"Carmela","family":"Giamp\u00e0","sequence":"first","affiliation":[{"name":"Istituto di Anatomia Umana e Biologia Cellulare, Universit\u00e0 Cattolica del Sacro Cuore Rome Italy"},{"name":"Fondazione Policlinico Universitario A. Gemelli IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Alvino","sequence":"additional","affiliation":[{"name":"Istituto di Anatomia Umana e Biologia Cellulare, Universit\u00e0 Cattolica del Sacro Cuore Rome Italy"},{"name":"Fondazione Policlinico Universitario A. Gemelli IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Magatti","sequence":"additional","affiliation":[{"name":"Centro di Ricerca E. Menni, Fondazione Poliambulanza Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonietta R.","family":"Silini","sequence":"additional","affiliation":[{"name":"Centro di Ricerca E. Menni, Fondazione Poliambulanza Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Cardinale","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroanatomy Santa Lucia Foundation IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Paldino","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroanatomy Santa Lucia Foundation IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca R.","family":"Fusco","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroanatomy Santa Lucia Foundation IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5211-6430","authenticated-orcid":false,"given":"Ornella","family":"Parolini","sequence":"additional","affiliation":[{"name":"Istituto di Anatomia Umana e Biologia Cellulare, Universit\u00e0 Cattolica del Sacro Cuore Rome Italy"},{"name":"Centro di Ricerca E. Menni, Fondazione Poliambulanza Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90585-E"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199805000-00001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3668"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000222734.56412.17"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2017.00193"},{"key":"e_1_2_7_7_1","first-page":"936","article-title":"Immunomodulatory strategies for Huntington\u2019s disease treatment","volume":"16","author":"Colpo G","year":"2017","journal-title":"CNS Neurol Disord Drug Targets"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu151"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13553"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.20018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000133403.30559.FF"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4008-12.2012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10818"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953-816X.2004.03372.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.23236"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712804485881"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009347"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2016.12"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2007.01.003"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02237-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.182682"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368916X693699"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1201\/b19620-6","volume-title":"Placenta tree life","author":"Magatti M","year":"2016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000001864"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2011.07.001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)02447-2"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci3020877"},{"key":"e_1_2_7_28_1","article-title":"Lysine and arginine reduce the effects of cerebral ischemic insults and inhibit glutamate\u2010induced neuronal activity in rats","volume":"4","author":"Kondoh T","year":"2010","journal-title":"Front Integr Neurosci"},{"key":"e_1_2_7_29_1","first-page":"52P","article-title":"The actions of certain amino acids as neuronal excitants [proceedings]","volume":"272","author":"Hall J","year":"1977","journal-title":"J Physiol"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60982-5"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13078219"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2014.2133"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00080.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.07112.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2014.07.004"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3584-0_13"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20680"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(03)00185-0"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.16.12.1933"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81369-0"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-07-02522.1999"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2017.02.076"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-174"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03102-5"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2010.10.015"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2007.06.012"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00144"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2012.04.008"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu6125453"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059581"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv218"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.083287"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00322"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1057"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(200001)47:1<80::AID-ANA13>3.0.CO;2-K"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/77528"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2015.02.005"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26192"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0248-1"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt341"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015842"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21788"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059438"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2015.04.007"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58644"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T08:17:40Z","timestamp":1694679460000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,25]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/jcmm.14113"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14113","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,25]]},"assertion":[{"value":"2018-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}