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Despite the unquestionable clinical significance, important aspects of the development of secondary tumours of the central nervous system are largely uncharacterized, including extravasation of metastatic cells through the blood\u2010brain barrier. By using transmission electron microscopy, here we followed interactions of cancer cells and brain endothelial cells during the adhesion, intercalation\/incorporation and transendothelial migration steps. We observed that brain endothelial cells were actively involved in the initial phases of the extravasation by extending filopodia\u2010like membrane protrusions towards the tumour cells. Melanoma cells tended to intercalate between endothelial cells and to transmigrate by utilizing the paracellular route. On the other hand, breast cancer cells were frequently incorporated into the endothelium and were able to migrate through the transcellular way from the apical to the basolateral side of brain endothelial cells. When co\u2010culturing melanoma cells with cerebral endothelial cells, we observed N\u2010cadherin enrichment at melanoma\u2010melanoma and melanoma\u2010endothelial cell borders. However, for breast cancer cells N\u2010cadherin proved to be dispensable for the transendothelial migration both in vitro and in vivo. Our results indicate that breast cancer cells are more effective in the transcellular type of migration than melanoma cells.<\/jats:p>","DOI":"10.1111\/jcmm.14156","type":"journal-article","created":{"date-parts":[[2019,2,3]],"date-time":"2019-02-03T05:10:55Z","timestamp":1549170655000},"page":"2619-2631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Paracellular and transcellular migration of metastatic cells through the cerebral endothelium"],"prefix":"10.1111","volume":"23","author":[{"given":"Hildegard","family":"Herman","sequence":"first","affiliation":[{"name":"Institute of Life Sciences Vasile Goldi\u015f Western University of Arad Arad Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csilla","family":"Fazakas","sequence":"additional","affiliation":[{"name":"Institute of Biophysics Biological Research Centre Hungarian Academy of Sciences Szeged 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