{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T09:20:43Z","timestamp":1791537643264,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,17]],"date-time":"2019-02-17T00:00:00Z","timestamp":1550361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470798"],"award-info":[{"award-number":["81470798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500404"],"award-info":[{"award-number":["81500404"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670787"],"award-info":[{"award-number":["81670787"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mesenteric adipose tissue (MAT) inflammation is associated with non\u2010alcoholic fatty liver disease (NAFLD), and immune cells play pivotal roles in the inflammation of adipose tissue. Here, we investigated the roles of MAT B lymphocytes in NAFLD. Mice fed with high\u2010fat diet (HFD) and normal diet (ND) were killed in time gradients (4, 8 and 12\u00a0weeks). Compared with ND\u2010fed mice, intra\u2010hepatic CD45<jats:sup>+<\/jats:sup>CD19<jats:sup>+<\/jats:sup> B lymphocytes increased after 4\u00a0weeks (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) of HFD feeding, and lasted until the 12th week, infiltrated earlier than CD45<jats:sup>+<\/jats:sup>CD3<jats:sup>+<\/jats:sup> T lymphocytes and CD45<jats:sup>+<\/jats:sup>F4\/80<jats:sup>+<\/jats:sup> macrophages. The mRNA expression of tumour necrosis factor (TNF)\u2010\u03b1, interleukin (IL)\u20106 and monocyte chemotactic protein (MCP)\u20101 decreased in MAT of B<jats:sup>null<\/jats:sup> HFD\u2010fed mice compared to that in wild\u2010type HFD\u2010fed mice, along with lesser macrophages. Mesenteric adipose tissue B cells from HFD\u2010fed mice promoted macrophage differentiation to type\u2010\u0399 macrophages and expression of pro\u2010inflammatory cytokines in vitro. Macrophages pre\u2010treated with MAT B cells from HFD\u2010fed mice showed elevated mRNA expression of IL\u20106 and TNF\u2010\u03b1 and declined IL\u201010 levels in adipocytes compared to ND MAT B cell pre\u2010treated macrophages. Besides, internal near\u2010infrared scanning and external transwell assay showed that HFD MAT B cells migrated to the liver more than ND MAT B cells. High\u2010fat diet MAT B cells induced higher MCP\u20101 and lower IL\u201010 expression in primary hepatocytes compared to ND MAT B cells in co\u2010culture experiment. These data indicate that B lymphocytes infiltrate early in MAT during the development of NAFLD, which may not only promote MAT inflammation by regulating macrophages but also migrate to the liver and induce hepatocytes inflammation.<\/jats:p>","DOI":"10.1111\/jcmm.14232","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:53:37Z","timestamp":1550451217000},"page":"3375-3385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Mesenteric adipose tissue B lymphocytes promote local and hepatic inflammation in non\u2010alcoholic fatty liver disease mice"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhe","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Peking University People's Hospital Beijing P.R. China"},{"name":"Clinical Center of Immune\u2010Mediated Digestive Diseases Peking University People's Hospital Beijing P.R. 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