{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:51:02Z","timestamp":1772160662655,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004434","name":"Universit\u00e0 degli Studi di Firenze","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Urothelium and Lamina Propria (LP) are considered an integrate sensory system which is able to control the detrusor activity. Complete supra\u2010sacral spinal cord lesions cause Neurogenic Detrusor Overactivity (NDO) whose main symptoms are urgency and incontinence. NDO therapy at first consists in anti\u2010muscarinic drugs; secondly, in intra\u2010vesical injection of botulinum toxin. However, with time, all the patients become insensitive to the drugs and decide for cystoplastic surgery. With the aim to get deeper in both NDO and drug's efficacy lack pathogenesis, we investigated the innervation, muscular and connective changes in NDO bladders after surgery by using morphological and quantitative methodologies. Bladder innervation showed a significant global loss associated with an increase in the nerve endings located in the upper LP where a neurogenic inflammation was also present. Smooth muscle cells (SMC) anomalies and fibrosis were found in the detrusor. The increased innervation in the ULP is suggestive for a sprouting and could condition NDO evolution and drug efficacy length. Denervation might cause the SMC anomalies responsible for the detrusor altered contractile activity and intra\u2010cellular traffic and favour the appearance of fibrosis. Inflammation might accelerate these damages. From the clinical point of view, an early anti\u2010inflammatory treatment could positively influence the disease fate.<\/jats:p>","DOI":"10.1111\/jcmm.14294","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:12:14Z","timestamp":1554336734000},"page":"4076-4087","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Nerve sprouting and neurogenic inflammation characterize the neurogenic detrusor overactive bladder of patients no longer responsive to drug therapies"],"prefix":"10.1111","volume":"23","author":[{"given":"Chiara","family":"Traini","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulio","family":"Del Popolo","sequence":"additional","affiliation":[{"name":"Department of Neuro\u2010Urology Careggi University Hospital Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria\u2010Simonetta","family":"Faussone\u2010Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Guasti","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Catarinicchia","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1060-5025","authenticated-orcid":false,"given":"Maria Giuliana","family":"Vannucchi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01368.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2003.10.031"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22635"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-016-8212-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2018.01.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2006.01.025"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.2003.04186.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)63951-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.05938.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2003.12.017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000169481.42259.54"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)63952-3"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.02.037"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13308"},{"key":"e_1_2_8_16_1","first-page":"697","article-title":"Some ultrastructural aspects of human pathological pelvi\u2010ureteric junction in idiopathic hydronephrosis","volume":"14","author":"Faussone\u2010Pellegrini MS","year":"1982","journal-title":"J Submicrosc Cytol"},{"key":"e_1_2_8_17_1","first-page":"217","article-title":"Ultrastructural modifications of the tunica muscularis in congenital obstruction of the upper urinary tract. 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