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Secreted angioinhibitory factor thrombospondin\u20101 (TSP\u20101) is an adhesive glycoprotein that mediates cell\u2010to\u2010cell and cell\u2010to\u2010matrix interactions and is associated with platelet aggregation, angiogenesis and tumorigenesis. We have found that the expression of TSP\u20101 is significantly lower in human pituitary corticotroph tumours compared with normal adenohypophysis. This study aims to elucidate the role of TSP\u20101 in regulating the tumour function of pituitary adenomas. Forced overexpression of TSP\u20101 in a murine AtT20 pituitary corticotroph tumour cell line decreased corticotroph precursor hormone proopiomelanocortin (POMC) transcription and adrenocorticotropic hormone (ACTH) secretion. Functional studies showed that TSP\u20101 overexpression in pituitary adenoma cells suppressed proliferation, migration and invasion. We have demonstrated that TSP\u20101 is a direct target of miR\u2010449c. Further study showed that miR\u2010449c activity enhanced tumorigenesis by directly inhibiting TSP\u20101 expression. Low expression of lncTHBS1, along with low expression of TSP\u20101, was associated with the high expression of miR\u2010449c in Cushing's disease patients. Furthermore, RNA\u2010immunoprecipitation associates miR\u2010449c with lncTHBS1 suggesting that lncTHBS1 might be a negative regulator of miR\u2010449c. Taken together, this study has demonstrated that lncTHBS1 might function as competing endogenous RNA for miR\u2010449c, which could suppress the development of Cushing's disease.<\/jats:p>","DOI":"10.1111\/jcmm.14297","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T03:09:45Z","timestamp":1556075385000},"page":"4097-4110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["TSP\u20101 is downregulated and inversely correlates with miR\u2010449c expression in Cushing's disease"],"prefix":"10.1111","volume":"23","author":[{"given":"Jie","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai P.R. 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