{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:43:25Z","timestamp":1790289805152,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871599"],"award-info":[{"award-number":["81871599"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671882"],"award-info":[{"award-number":["81671882"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700259"],"award-info":[{"award-number":["81700259"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870186"],"award-info":[{"award-number":["81870186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009330","name":"Medical Science and Technology Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2015A020212012"],"award-info":[{"award-number":["2015A020212012"]}],"id":[{"id":"10.13039\/501100009330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009330","name":"Medical Science and Technology Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A020224012"],"award-info":[{"award-number":["2017A020224012"]}],"id":[{"id":"10.13039\/501100009330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2016A030311039"],"award-info":[{"award-number":["2016A030311039"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vascular progenitor cells (VPCs) present in the adventitia of the vessel wall play a critical role in the regulation of vascular repair following injury. This study aimed to assess the function of VPCs isolated from patients with Marfan syndrome (MFS). VPCs were isolated from control and MFS donors and characterized. Compared with control\u2010VPCs, MFS\u2010VPCs exhibited cellular senescence as demonstrated by increased cell size, higher SA\u2010\u03b2\u2010gal activity and elevated levels of p53 and p21. RNA sequencing showed that several cellular process\u2010related pathways including cell cycle and cellular senescence were significantly enriched in MFP\u2010VPCs. Notably, the expression level of TGF\u2010\u03b21 was much higher in MFS\u2010VPCs than control\u2010VPCs. Treatment of control\u2010VPCs with TGF\u2010\u03b21 significantly enhanced mitochondrial reactive oxidative species (ROS) and induced cellular senescence whereas inhibition of ROS reversed these effects. MFS\u2010VPCs displayed increased mitochondrial fusion and decreased mitochondrial fission. Treatment of control\u2010VPCs with TGF\u2010\u03b21 increased mitochondrial fusion and reduced mitochondrial fission. Nonetheless, treatment of mitofusin2 (Mfn2)\u2010siRNA inhibited TGF\u2010\u03b21\u2010induced mitochondrial fusion and cellular senescence. Furthermore, TGF\u2010\u03b21\u2010induced mitochondrial fusion was mediated by the AMPK signalling pathway. Our study shows that TGF\u2010\u03b21 induces VPC senescence in patients with MFS by mediating mitochondrial dynamics via the AMPK signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.14301","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T13:13:39Z","timestamp":1553778819000},"page":"4139-4152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Vascular progenitor cell senescence in patients with Marfan syndrome"],"prefix":"10.1111","volume":"23","author":[{"given":"Haiwei","family":"He","sequence":"first","affiliation":[{"name":"School of Medicine South China University of Technology Guangzhou China"},{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoqi","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"},{"name":"Department of Physiology and Pathophysiology School of Basic Medical Sciences Capital Medical University Key Laboratory of Remodelling\u2010Related Cardiovascular Diseases Ministry of Education Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Genomic Sciences LKS Faculty of Medicine the University of Hong Kong Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"You","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimei","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology Guangzhou China"},{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qizhou","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Medicine LKS Faculty of Medicine the University of Hong Kong Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0469-5121","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology Guangzhou China"},{"name":"Department of Emergency Medicine Department of Emergency and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/352337a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.21037\/acs.2017.11.09"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2013-304709"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309852"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.307303"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.221549"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.300902"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.302568"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5404"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2017.0078"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.841981"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2017.10.003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.300675"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2018.01.030"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2016-0089"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.09.023"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1722452115"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19628"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.91"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309853"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-016-0278-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-018-0567-y"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12962"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.312945"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.04.055"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0970-6"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3565127"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13220"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-018-0584-7"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab4138"},{"key":"e_1_2_8_32_1","first-page":"681","article-title":"Vascular wall\u2010resident stem cells","volume":"25","author":"Klein D","year":"2010","journal-title":"Histol Histopathol"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.sj.6300145"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7171"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303809"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.02.023"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.11.015"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-015-0241-6"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.05.054"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700679200"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2016.00299"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox7010007"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.02.017"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.11.011"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1723"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2017.07.004"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14301","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T19:06:10Z","timestamp":1694372770000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,28]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/jcmm.14301"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14301","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,28]]},"assertion":[{"value":"2018-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}