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Adenosine monophosphate\u2010activated protein kinase (AMPK) signalling is widely recognized exerting cardioprotective effect in various cardiomyopathy. Here, we aimed to investigate whether the protective effects of the CAP are associated with AMPK signalling in ICM. In vivo, coronary artery of rats was ligated for 4\u00a0weeks to induce the ICM and then treated with PNU\u2010282987 (CAP agonist) and BML\u2010275 dihydrochloride (AMPK antagonist) for 4\u00a0weeks. In vitro, primary macrophages harvested from rats were induced inflammation by Lipopolysaccharide (LPS) treatment and then treated with PNU\u2010282987 and BML\u2010275 dihydrochloride. In vivo, exciting CAP by PUN\u2010282987 elicited an activation of AMPK signalling, alleviated ventricular remodeling, modified the cardiac electrophysiological function, reduced the cardiac expression of collagens and inflammatory cytokines and maintained the integrity of ultrastructure in the ischemic heart. However, the benefits of CAP excitation were blunted by AMPK signaling antagonization. In vitro, excitation of the CAP was observed inhibiting the nuclear transfer of NF\u2010\u03baB p65 of macrophages and promoting the transformation of Ly\u20106C<jats:sup>high<\/jats:sup> macrophages into Ly\u20106C<jats:sup>low<\/jats:sup> macrophages. However, inhibiting AMPK signalling by BML\u2010275 dihydrochloride reversed the CAP effect on LPS\u2010treated macrophages. Finally, our findings suggest that eliciting the CAP modulates the inflammatory response in ICM through regulating AMPK signalling.<\/jats:p>","DOI":"10.1111\/jcmm.14363","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T05:59:50Z","timestamp":1557208790000},"page":"4746-4758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Eliciting \u03b17\u2010nAChR exerts cardioprotective effects on ischemic cardiomyopathy via activation of AMPK signalling"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhong\u2010Hao","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Cardiology Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue\u2010Chun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology Second Affiliated 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