{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:24:22Z","timestamp":1790508262636,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,11]],"date-time":"2019-08-11T00:00:00Z","timestamp":1565481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,11]],"date-time":"2019-08-11T00:00:00Z","timestamp":1565481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Osteoarthritis (OA) is a prevalent degenerative joint disease whose pathogenesis remains unclear. The research aims to investigate the roles of Circ_0136474\/miR\u2010127\u20105p\/MMP\u201013 axis in OA. Differentially expressed circRNAs and miRNAs in OA cartilage tissue were screened out and visualized by R project based on RNA\u2010seq data and microarray data respectively. qRT\u2010PCR was carried out for detection of relative expression levels of Circ_0136474, miR\u2010127\u20105p, MMP\u201013 and other inflammatory factors and Western blot analysis was conducted to detect the protein expression level of MMP\u201013. CCK\u20108 assay and flow cytometry were conducted to determine cell proliferation and cell apoptotic ability respectively. RNA\u2010fluorescence in situ hybridization (RNA\u2010FISH) experiments were conducted to confirm the immune\u2010localization of the Circ_0136474 and MMP\u201013 in human tissues. Targeted relationships were predicted by bioinformatic analysis and verified by dual\u2010luciferase reporter assay. Our findings revealed that the expression levels of both Circ_0136474 and MMP\u201013 in OA cartilage tissue were significantly higher than that in normal cartilage tissue. Circ_0136474 could suppress cell proliferation by facilitating MMP\u201013 expression and suppressing miR\u2010127\u20105p expression in OA. Overexpression of miR\u2010127\u20105p negatively regulated MMP\u201013 expression to enhance cell proliferation. Our study demonstrated that Circ_0136474 and MMP\u201013 suppressed cell proliferation, while enhanced cell apoptosis by competitive binding to miR\u2010127\u20105p in OA, which may well provide us with a new therapeutic strategy for osteoarthritis.<\/jats:p>","DOI":"10.1111\/jcmm.14400","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T02:15:54Z","timestamp":1565576154000},"page":"6554-6564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Circ_0136474 and MMP\u201013 suppressed cell proliferation by competitive binding to miR\u2010127\u20105p in osteoarthritis"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhao","family":"Li","sequence":"first","affiliation":[{"name":"Center of Arthritis Clinic &amp; Research Peking University People\u2019s Hospital Beijing China"},{"name":"Department of Orthopedics Peking University Shougang Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Civil Aviation General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Peking University Shougang Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keshi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Arthritis Clinic &amp; 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