{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:11:03Z","timestamp":1787015463223,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T00:00:00Z","timestamp":1559088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FU734\/4-1"],"award-info":[{"award-number":["FU734\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Primary open\u2010angle glaucoma (POAG) is one of the most common causes for blindness worldwide. Although an elevated intraocular pressure (IOP) is the main risk factor, the exact pathology remained indistinguishable. Therefore, it is necessary to have appropriate models to investigate these mechanisms. Here, we analysed a transgenic glaucoma mouse model (\u03b2B1\u2010CTGF) to elucidate new possible mechanisms of the disease. Therefore, IOP was measured in \u03b2B1\u2010CTGF and wildtype mice at 5, 10 and 15\u00a0weeks of age. At 5 and 10\u00a0weeks, the IOP in both groups were comparable (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&gt;\u00a00.05). After 15\u00a0weeks, a significant elevated IOP was measured in \u03b2B1\u2010CTGF mice (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). At 15\u00a0weeks, electroretinogram measurements were performed and both the a\u2010 and b\u2010wave amplitudes were significantly decreased in \u03b2B1\u2010CTGF retinae (both\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). Significantly fewer Brn\u20103a\n                    <jats:sup>+<\/jats:sup>\n                    retinal ganglion cells (RGCs) were observed in the \u03b2B1\u2010CTGF group on flatmounts (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.02), cross\u2010sections (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) and also via quantitative real\u2010time PCR (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.02). Additionally, significantly more cleaved caspase 3\n                    <jats:sup>+<\/jats:sup>\n                    RGCs were seen in the \u03b2B1\u2010CTGF group (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.002). Furthermore, a decrease in recoverin\n                    <jats:sup>+<\/jats:sup>\n                    cells was observable in the \u03b2B1\u2010CTGF animals (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.004). Accordingly, a significant down\u2010regulation of\n                    <jats:italic>Recoverin<\/jats:italic>\n                    mRNA levels were noted (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001).\n                    <jats:italic>Gfap<\/jats:italic>\n                    expression, on the other hand, was higher in \u03b2B1\u2010CTGF retinae (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.023). Additionally, more glutamine synthetase signal was noted (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.04). Although no alterations were observed regarding photoreceptors via immunohistology, a significant decrease of\n                    <jats:italic>Rhodopsin<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.003) and\n                    <jats:italic>Opsin<\/jats:italic>\n                    mRNA (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.03) was noted. We therefore assume that the \u03b2B1\u2010CTGF mouse could serve as an excellent model for better understanding the pathomechanisms in POAG.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14433","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T01:09:18Z","timestamp":1559178558000},"page":"5497-5507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Loss of retinal ganglion cells in a new genetic mouse model for primary open\u2010angle glaucoma"],"prefix":"10.1111","volume":"23","author":[{"given":"Sabrina","family":"Reinehr","sequence":"first","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Koch","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Weiss","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Froemel","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Voss","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Burkhard","family":"Dick","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Fuchshofer","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7056-0829","authenticated-orcid":false,"given":"Stephanie C.","family":"Joachim","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2016-EGSguideline.002"},{"key":"e_1_2_8_3_1","first-page":"741","article-title":"Number of ganglion cells in glaucoma eyes compared with threshold visual field tests in the same persons","volume":"41","author":"Kerrigan\u2010Baumrind LA","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0184"},{"key":"e_1_2_8_5_1","first-page":"402","article-title":"Translimbal laser photocoagulation to the trabecular meshwork as a model of glaucoma in rats","volume":"43","author":"Levkovitch\u2010Verbin H","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(05)80131-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3947"},{"key":"e_1_2_8_8_1","first-page":"951","article-title":"Essential iris atrophy, pigment dispersion, and glaucoma in DBA\/2J mice","volume":"39","author":"John SW","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523805225130"},{"key":"e_1_2_8_10_1","first-page":"211","article-title":"Characterization of intraocular pressure pattern and changes of retinal ganglion cells in DBA2J glaucoma mice","volume":"9","author":"Wang J","year":"2016","journal-title":"Int J Ophthalmol"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-4-20"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.6.080604.162209"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.02.030"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3210-09.2010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-015-0707-2"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040616"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-13372"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1208-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2016.00140"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-017-2710-0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-18999R2"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43470"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061636"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12909"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(92)31774-9"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1991.03470030069026"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(94)31090-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2008.08.006"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2010.01.034"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.04.003"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0077"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(96)30449-1"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/00061198-200312000-00001"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5420.1670"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605342103"},{"key":"e_1_2_8_37_1","unstructured":"Open Angle Glaucoma \u2010 Diagnosis Follow\u2010up and Treatment: A Systematic Literature Review. 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