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The latter is a more complex diabetic complication in which neurodegeneration plays a significant role and a leading cause of blindness. The vascular endothelial growth factor (VEGF) is a powerful pro\u2010angiogenic factor that acts through three tyrosine kinase receptors (VEGFR\u20101, VEGFR\u20102 and VEGFR\u20103). In this work we studied the anti\u2010angiogenic effect of quercetin (Q) and some of its derivates in human microvascular endothelial cells, as a blood retinal barrier model, after stimulation with VEGF\u2010A. We found that a permethylated form of Q, namely 8MQPM, more than the simple Q, is a potent inhibitor of angiogenesis both in vitro and ex vivo. Our results showed that these compounds inhibited cell viability and migration and disrupted the formation of microvessels in rabbit aortic ring. The addition of Q and more significantly 8MQPM caused recoveries or completely re\u2010establish the transendothelial electrical resistance (TEER) to the control values and suppressed the activation of VEGFR2 downstream signalling molecules such as AKT, extracellular signal\u2010regulated kinase, and c\u2010Jun N\u2010terminal kinase. Taken together, these data suggest that 8MQPM might have an important role in the contrast of angiogenesis\u2010related diseases.<\/jats:p>","DOI":"10.1111\/jcmm.14455","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T09:31:05Z","timestamp":1564651865000},"page":"6565-6577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Anti\u2010angiogenic effect of quercetin and its 8\u2010methyl pentamethyl ether derivative in human microvascular endothelial cells"],"prefix":"10.1111","volume":"23","author":[{"given":"Gabriella","family":"Lupo","sequence":"first","affiliation":[{"name":"Section of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences (Biometec), School of Medicine University of Catania Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Cambria","sequence":"additional","affiliation":[{"name":"Section 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