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However, little is known about its effect on Crohn's disease (CD). We aimed to investigate the impact of Bry\u20101 on CD\u2010like colitis and determine the mechanism underlying this effect. In the present study, 15\u2010week\u2010old male <jats:italic>Il\u201010<\/jats:italic><jats:sup>\u2212\/\u2212 <\/jats:sup>mice with spontaneous colitis were divided into positive control and Bry\u20101\u2010treated (Bry\u20101, 30\u00a0\u03bcg\/kg every other day, injected intraperitoneally for 4\u00a0weeks) groups. Age\u2010matched, male wild\u2010type (WT) mice were used as a negative control. The effects of Bry\u20101 on colitis, intestinal barrier function and T cell responses as well as the potential regulatory mechanisms were evaluated. We found that the systemic delivery of Bry\u20101 significantly ameliorated colitis in <jats:italic>Il\u201010<\/jats:italic><jats:sup>\u2212\/\u2212 <\/jats:sup>mice, as demonstrated by decreases in the disease activity index (DAI), inflammatory score and proinflammatory mediator levels. The protective effects of Bry\u20101 on CD\u2010like colitis included the maintenance of intestinal barrier integrity and the helper T cell (Th)\/regulatory T cell (Treg) balance. These effects of Bry\u20101 may act in part through nuclear factor erythroid 2\u2010related factor 2 (Nrf2) signalling activation and STAT3\/4 signalling inhibition. The protective effect of Bry\u20101 on CD\u2010like colitis suggests Bry\u20101 has therapeutic potential in human CD, particularly given the established clinical safety of Bry\u20101.<\/jats:p>","DOI":"10.1111\/jcmm.14457","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T13:49:58Z","timestamp":1561729798000},"page":"5588-5599","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Bryostatin\u20101 ameliorated experimental colitis in <i>Il\u201010<\/i><sup>\u2212\/\u2212<\/sup> Mice by protecting the intestinal barrier and limiting immune dysfunction"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0084-2681","authenticated-orcid":false,"given":"Lugen","family":"Zuo","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery First Affiliated Hospital of Bengbu Medical College Bengbu China"},{"name":"Anhui Key Laboratory of 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