{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T04:47:47Z","timestamp":1774673267829,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T00:00:00Z","timestamp":1565395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T00:00:00Z","timestamp":1565395200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Science and Technology Development Plan Project of Jilin Provincial Science and Technology Department","award":["20160101032JC"],"award-info":[{"award-number":["20160101032JC"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, our aim was to exploring the influences of DNA methylation of\n                    <jats:italic>PON1<\/jats:italic>\n                    on cell proliferation, migration and apoptosis of renal cancer cells. The genome\u2010wide methylation array of renal cell carcinoma samples and adjacent tissues were obtained from the cancer genome atlas (TCGA) database. By analysing the DNA methylation and conducting the CpG islands array, methylation status expressed in renal tumour samples and normal renal tissue samples were detected. Methylation\u2010specific PCR (MS\u2010PCR) and qRT\u2010PCR were employed to detect the methylation level and mRNA expression of\n                    <jats:italic>PON1<\/jats:italic>\n                    . Wound\u2010healing assay, transwell assay and MTT assay were utilized to detecting the migration, invasion and proliferation abilities, respectively. The cell apoptosis was testified by Tunnel assay. In addition, the effect of\n                    <jats:italic>PON1<\/jats:italic>\n                    on renal cancer cells was verified by experiments in vivo. The methylation status of different genes in renal cell carcinoma samples was obtained by CpG islands arrays and hypermethylated\n                    <jats:italic>PON1<\/jats:italic>\n                    was selected for further study.\n                    <jats:italic>PON1<\/jats:italic>\n                    was down\u2010regulated in renal cell carcinoma tissues detected by qRT\u2010PCR and Western blot. Both in vitro and vivo experiments indicated that the sunitinib\u2010resistant in renal cancer cells could be suppressed by treat with 5\u2010Aza\u2010dC or TSA, and the effect came out more obvious after 5\u2010Aza\u2010dC and TSA co\u2010treatment. In detail, the demethylation of\n                    <jats:italic>PON1<\/jats:italic>\n                    inhibited the migration, invasion and proliferation of renal cancer cells and also arrested more cells in G0\/G1 phase. The\n                    <jats:italic>vivo<\/jats:italic>\n                    experiment indicated that demethylated\n                    <jats:italic>PON1<\/jats:italic>\n                    suppressed the growth of tumour. Hypermethylated\n                    <jats:italic>PON1<\/jats:italic>\n                    promoted migration, invasion and proliferation of sunitinib\u2010resistance renal cancer cells and arrested more cells in G0\/G1 phase.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14537","type":"journal-article","created":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T03:10:11Z","timestamp":1565406611000},"page":"6646-6657","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>PON1<\/i>\n                    hypermethylation is associated with progression of renal cell carcinoma"],"prefix":"10.1111","volume":"23","author":[{"given":"Xin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy China\u2010Japan Union Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7055-164X","authenticated-orcid":false,"given":"Qian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy China\u2010Japan Union Hospital of Jilin University Changchun 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