{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:47:52Z","timestamp":1791920872697,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,21]],"date-time":"2019-07-21T00:00:00Z","timestamp":1563667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,21]],"date-time":"2019-07-21T00:00:00Z","timestamp":1563667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772360 and 31771106"],"award-info":[{"award-number":["81772360 and 31771106"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Osteoclast overactivation\u2010induced imbalance in bone remodelling leads to pathological bone destruction, which is a characteristic of many osteolytic diseases such as rheumatoid arthritis, osteoporosis, periprosthetic osteolysis and periodontitis. Natural compounds that suppress osteoclast formation and function have therapeutic potential for treating these diseases. Stachydrine (STA) is a bioactive alkaloid isolated from\n                    <jats:italic>Leonurus heterophyllus<\/jats:italic>\n                    Sweet and possesses antioxidant, anti\u2010inflammatory, anticancer and cardioprotective properties. However, its effects on osteoclast formation and function have been rarely described. In the present study, we found that STA suppressed receptor activator of nuclear factor\u2010\u03baB (NF\u2010\u03baB) ligand (RANKL)\u2010induced osteoclast formation and bone resorption, and reduced osteoclast\u2010related gene expression in vitro. Mechanistically, STA inhibited RANKL\u2010induced activation of NF\u2010\u03baB and Akt signalling, thus suppressing nuclear factor of activated T cells c1 induction and nuclear translocation. In addition, STA alleviated bone loss and reduced osteoclast number in a murine model of LPS\u2010induced inflammatory bone loss. STA also inhibited the activities of NF\u2010\u03baB and NFATc1 in vivo. Together, these results suggest that STA effectively inhibits osteoclastogenesis both in vitro and in vivo and therefore is a potential option for treating osteoclast\u2010related diseases.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14551","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T00:39:56Z","timestamp":1563755996000},"page":"6730-6743","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Stachydrine prevents LPS\u2010induced bone loss by inhibiting osteoclastogenesis via NF\u2010\u03baB and Akt signalling"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9091-1745","authenticated-orcid":false,"given":"Jiahong","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, The Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Orthopedic Research Institute of 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