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The present study was conducted to explore CaSR\/NLRP3 inflammasome pathway activation and cytokines IL\u20101\u03b2 and IL\u201018 release in oesophageal epithelia injured by refluxates and the effects of Tojapride on that signal regulation. Using a modified RE rat model with Tojapride administration and Tojapride\u2010pretreated SV40\u2010immortalized human oesophageal epithelial cells (HET\u20101A) exposed to acidic bile salts pretreated with Tojapride, we evaluated the therapeutic effects of Tojapride on oesophageal epithelial barrier function, the expression of CaSR\/NLRP3 inflammasome pathway\u2010related proteins and the release of downstream cytokines in response to acidic bile salt irritation. In vivo, Tojapride treatment ameliorated the general condition and pathological lesions of the oesophageal epithelium in modified RE rats. In addition, Tojapride effectively blocked the CaSR\u2010mediated NLRP3 inflammasome activation in modified RE rats. In vitro, Tojapride treatment can reverse the harmful effect of acidic bile salts, which reduced transepithelial electrical resistance (TEER), up\u2010regulated the CaSR\u2010mediated NLRP3 inflammasome pathway and increased caspase\u20101 activity, LDH release and cytokines secretion. Taken together, these data show that Tojapride can prevent CaSR\u2010mediated NLRP3 inflammasome activation and alleviate oesophageal epithelial injury induced by acidic bile salt exposure.<\/jats:p>","DOI":"10.1111\/jcmm.14631","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T07:41:33Z","timestamp":1576827693000},"page":"1208-1219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Tojapride prevents CaSR\u2010mediated NLRP3 inflammasome activation in oesophageal epithelium irritated by acidic bile salts"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6563-0685","authenticated-orcid":false,"given":"Xiao\u2010Lan","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Gastroenterology China Academy of Chinese Medical Sciences Xiyuan Hospital Beijing 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