{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:01:39Z","timestamp":1790434899959,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370219 and 81900258"],"award-info":[{"award-number":["81370219 and 81900258"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stromal cell\u2010derived factor\u20101 (SDF\u20101) is a well\u2010characterized cytokine that protects heart from ischaemic injury. However, the beneficial effects of native SDF\u20101, in terms of promoting myocardial repair, are limited by its low concentration in the ischaemic myocardium. Annexin V (AnxA5) can precisely detect dead cells in vivo. As massive cardiomyocytes die after MI, we hypothesize that AnxA5 can be used as an anchor to carry SDF\u20101 to the ischaemic myocardium. In this study, we constructed a fusion protein consisting of SDF\u20101 and AnxA5 domains. The receptor competition assay revealed that SDF\u20101\u2010AnxA5 had high binding affinity to SDF\u20101 receptor CXCR4. The treatment of SDF\u20101\u2010AnxA5 could significantly promote phosphorylation of AKT and ERK and induce chemotactic response, angiogenesis and cell survival in vitro. The binding membrane assay and immunofluorescence revealed that AnxA5 domain had the ability to specifically recognize and bind to cells injured by hypoxia. Furthermore, SDF\u20101\u2010AnxA5 administered via peripheral vein could accumulate at the infarcted myocardium in vivo. The treatment with SDF\u20101\u2010AnxA5 attenuated cell apoptosis, enhanced angiogenesis, reduced infarcted size and improved cardiac function after mouse myocardial infarction. Our results suggest that the bifunctional SDF\u20101\u2010AnxA5 can specifically bind to dead cells. The systemic administration of bifunctional SDF\u20101\u2010AnxA5 effectively provides cardioprotection after myocardial infarction.<\/jats:p>","DOI":"10.1111\/jcmm.14640","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T07:50:40Z","timestamp":1567151440000},"page":"7673-7684","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["The bifunctional SDF\u20101\u2010AnxA5 fusion protein protects cardiac function after myocardial infarction"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3049-2996","authenticated-orcid":false,"given":"Fang\u2010Yang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"},{"name":"State Key Laboratory of Biotherapy and Cancer Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian\u2010Li","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Li","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Diseases Regenerative Medicine Research Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010Gang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hua","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Diseases Regenerative Medicine Research Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Biao","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"},{"name":"Laboratory of Cardiovascular Diseases Regenerative Medicine Research Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Diseases Regenerative Medicine Research Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Biomaterials Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Diseases Regenerative Medicine Research Center West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.300215"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2012.32"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.070508"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302884"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.694992"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-005-0521-z"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.197723"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6558com"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.198929"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.55"},{"key":"e_1_2_8_12_1","first-page":"1536","article-title":"Detection of cardiomyocyte death in a rat model of ischemia and reperfusion using 99mTc\u2010labeled annexin V","volume":"45","author":"Taki J","year":"2004","journal-title":"J Nucl Med"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2012.719898"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10969-005-2827-3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.37.21594"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.107"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16813"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08840-y"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.672451"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.02.013"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt181"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9701"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.033703"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2008.00519.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000147609.39780.02"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.718718"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000343"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26683"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000147780.30124.CF"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv254"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14232-8"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.068429"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.13.1564"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13550-015-0152-9"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.03.016"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21350"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/561098"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.01.024"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14640","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14640","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14640","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14640","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T03:54:16Z","timestamp":1692244456000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,30]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/jcmm.14640"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14640","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,30]]},"assertion":[{"value":"2019-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}