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Long non\u2010coding RNAs (lncRNAs) are emerging as a new player in gene regulation relevant to a wide spectrum of human disease including cardiac disorders. Here, we characterize the role of a specific lncRNA named cardiac hypertrophy\u2010associated regulator (CHAR) in CH and delineate the underlying signalling pathway. CHAR was found markedly down\u2010regulated in both in vivo mouse model of cardiac hypertrophy induced by pressure overload and in vitro cellular model of cardiomyocyte hypertrophy induced by angiotensin II (AngII) insult. CHAR down\u2010regulation alone was sufficient to induce hypertrophic phenotypes in healthy mice and neonatal rat ventricular cells (NRVCs). Overexpression of CHAR reduced the hypertrophic responses. CHAR was found to act as a competitive endogenous RNA (ceRNA) to down\u2010regulate miR\u201020b that we established as a pro\u2010hypertrophic miRNA. We experimentally established phosphatase and tensin homolog (PTEN), an anti\u2010hypertrophic signalling molecule, as a target gene for miR\u201020b. We found that miR\u201020b induced CH by directly repressing PTEN expression and indirectly increasing AKT activity. Moreover, CHAR overexpression mitigated the repression of PTEN and activation of AKT by miR\u201020b, and as such, it abrogated the deleterious effects of miR\u201020b on CH. Collectively, this study characterized a new lncRNA CHAR and unravelled a new pro\u2010hypertrophic signalling pathway: lncRNA\u2010CHAR\/miR\u201020b\/PTEN\/AKT. The findings therefore should improve our understanding of the cellular functionality and pathophysiological role of lncRNAs in the heart.<\/jats:p>","DOI":"10.1111\/jcmm.14641","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T16:00:59Z","timestamp":1567094459000},"page":"7685-7698","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Long non\u2010coding RNA cardiac hypertrophy\u2010associated regulator governs cardiac hypertrophy via regulating miR\u201020b and the downstream PTEN\/AKT pathway"],"prefix":"10.1111","volume":"23","author":[{"given":"Mingyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology (State\u2010Province Key Laboratories of Biomedicine\u2010Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education) College of Pharmacy Harbin Medical University Harbin 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