{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:15:29Z","timestamp":1783462529304,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth science fund project","award":["81700470"],"award-info":[{"award-number":["81700470"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the efficacy of sacral nerve stimulation (SNS) on nerve growth factor (NGF) mediated visceral sensitivity in normal rat and visceral hypersensitivity model rats. 120 male newborn rats were randomly divided into 6 groups: group A was normal model group; group B\u00a0~\u00a0F were all sensitized with acetic acid enema and grouped again. Group c2 was given NGF antagonist, d2 group was given NGF agonist, e2 group was given PI3K inhibitor, and f2 group was given PLC\u2010\u03b3 inhibitor. After treatment, the expression of NGF, TrKA, PI3K, AKT, PLC\u2010\u03b3, NF\u2010\u03baB, TRPV1, pTRPV1 and intracellular Ca<jats:sup>2+<\/jats:sup> content were detected. The expression of protein TRPV1 and pTRPV1 was increased, and Ca<jats:sup>2+<\/jats:sup> was increased in the visceral hypersensitive group. NGF, TrKA in NGF antagonist group, PI3K, AKT, NF\u2010\u03baB in PI3K inhibitor group, PLC\u2010\u03b3 in PLC\u2010\u03b3 inhibitor group were all almost not expressed. The relative expression of NGF, TrKA, PI3K, AKT, PLC\u2010\u03b3 and NF\u2010\u03baB in NGF antagonist group was lower than that in visceral hypersensitivity group and NGF activator group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). The relative expression of NGF, TrKA, PI3K and AKT mRNA in NGF antagonist group was lower than that in the normal model group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). There was no significant difference in the relative expression of PLC\u2010\u03b3 and NF\u2010\u03baB mRNA (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05). The expression level of MAPK, ERK1 and ERK2 in visceral hypersensitivity group was higher than that in PI3K inhibitor group and PLC\u2010\u03b3 inhibitor group. The normal group Ca<jats:sup>2+<\/jats:sup> curve was flat, and the NGF agonist group had the highest Ca<jats:sup>2+<\/jats:sup> curve peak. Calcium concentration in visceral hypersensitivity group was higher than that in PI3K inhibitor group and that in PLC\u2010\u03b3 inhibitor group was higher than that in NGF antagonist group. The binding of TrkA receptor to NGF activates the MAPK\/ERK pathway, the PI3K\/Akt pathway and the PLC\u2010\u03b3 pathway, causing changes in the fluidity of intracellular and extracellular Ca<jats:sup>2+<\/jats:sup>, resulting in increased sensitivity of visceral tissues and organs.<\/jats:p>","DOI":"10.1111\/jcmm.14660","type":"journal-article","created":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T10:07:10Z","timestamp":1571738830000},"page":"8019-8024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect and mechanisms of sacral nerve stimulation on visceral hypersensitivity mediated by nerve growth factor"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0567-9182","authenticated-orcid":false,"given":"Liuqin","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The First Affiliated Hospital of Nanjing Medical University 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