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In this study, we aimed to investigate a novel approach to exhibit effective haemostatic properties, which could possibly save lives in various bleeding emergencies. According to the structure\u2010based enzymatic design, we have engineered a novel single\u2010chain hybrid enzyme complex (SCHEC), COX\u20101\u201010aa\u2010TXAS. We linked the C\u2010terminus of cyclooxygenase\u20101 (COX\u20101) to the N\u2010terminus of the thromboxane A<jats:sub>2<\/jats:sub> (TXA<jats:sub>2<\/jats:sub>) synthase (TXAS), through a 10\u2010amino acid residue linker. This recombinant COX\u20101\u201010aa\u2010TXAS can effectively pass COX\u20101\u2013derived intermediate prostaglandin (PG) H<jats:sub>2<\/jats:sub> (PGH<jats:sub>2<\/jats:sub>) to the active site of TXAS, resulting in an effective chain reaction property to produce the haemostatic prostanoid, TXA<jats:sub>2<\/jats:sub>, rapidly. Advantageously, COX\u20101\u201010aa\u2010TXAS constrains the production of other pro\u2010bleeding prostanoids, such as prostacyclin (PGI<jats:sub>2<\/jats:sub>) and prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>), through reducing the common substrate, PGH<jats:sub>2<\/jats:sub> being passed to synthases which produce aforementioned prostanoids. Therefore, based on these multiple properties, this novel COX\u20101\u201010aa\u2010TXAS indicated a powerful anti\u2010bleeding ability, which could be used to treat a variety of bleeding situations and could even be useful for bleeding prone situations, including nonsteroidal anti\u2010inflammatory drugs (NSAIDs)\u2010resulted TXA<jats:sub>2<\/jats:sub>\u2010deficient and PGI<jats:sub>2<\/jats:sub>\u2010mediated bleeding disorders. This novel SCHEC has a great potential to be developed into a biological haemostatic agent to treat severe haemorrhage emergencies, which will prevent the complications of blood loss and save lives.<\/jats:p>","DOI":"10.1111\/jcmm.14711","type":"journal-article","created":{"date-parts":[[2019,10,19]],"date-time":"2019-10-19T08:40:33Z","timestamp":1571474433000},"page":"8343-8354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A novel single\u2010chain enzyme complex with chain reaction properties rapidly producing thromboxane A<sub>2<\/sub> and exhibiting powerful anti\u2010bleeding functions"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9741-1185","authenticated-orcid":false,"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacological and Pharmaceutical Sciences Center for Experimental Therapeutics and Pharmacoinformatics College of Pharmacy University of Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun\u2010Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacological and Pharmaceutical Sciences Center for Experimental Therapeutics and Pharmacoinformatics College of Pharmacy University of Houston Houston TX USA"},{"name":"Visiting Scholar from Department of Ultrasound Second Affiliated Hospital Zhejiang University College of Medicine Hangzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Lan","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Pharmacological and Pharmaceutical Sciences Center for Experimental Therapeutics and Pharmacoinformatics College of Pharmacy University of Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shui\u2010Ping","family":"So","sequence":"additional","affiliation":[{"name":"Department of 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