{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:20:33Z","timestamp":1781101233479,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772776"],"award-info":[{"award-number":["81772776"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772785"],"award-info":[{"award-number":["81772785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872115"],"award-info":[{"award-number":["81872115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ovarian cancer is a leading cause of deaths due to gynaecological malignancy. While endogenous non\u2010coding circular RNAs (circRNAs) in cancer have attracted attention, their roles in ovarian cancer are not known. We used qRT\u2010PCR to quantify expression of circRhoC in ovarian cancer tissues and normal tissues. The effects of overexpressing or destruction of circRhoC on the phenotype of ovarian cancer cells were assessed both in vitro and in vivo. Dual\u2010luciferase reporter assay assesses the microRNA sponge function of circRhoC. Western blotting was used to confirm the effects of circRhoC and microRNA on target gene expression. Our results showed that circRhoC was significantly up\u2010regulated in ovarian cancer tissues compared to normal ovarian tissues. Overexpression of circRhoC in CAOV3 ovarian cancer cell increased cell viability, migration and invasion ability; destroying circRhoC in A2780 had the opposite effects and inhibited ovarian tumour cell A2780 dissemination in the peritoneum in vivo. We confirmed circRhoC functions as a sponge for miR\u2010302e to positively regulate <jats:italic>VEGFA<\/jats:italic>; FISH experiments showed that circRhoC could co\u2010focal with miR\u2010302e; besides, overexpression of miR\u2010302e reversed the ability of circRhoC to positively regulate VEGFA, and what's more, RIP assay showed that circRhoC could directly bind with VEGFA; besides, VEGFA expression level in ovarian cancer tissues was positively associated with circRhoC expression. In conclusion, the oncogenic effect of RhoC in ovarian cancer is at least in part due to circRhoC, which functions not only as a miR\u2010302e sponge to positively regulate VEGFA protein expression, but may also directly bind and modulate VEGFA expression.<\/jats:p>","DOI":"10.1111\/jcmm.14736","type":"journal-article","created":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T16:04:18Z","timestamp":1571760258000},"page":"8472-8481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["CircRhoC promotes tumorigenicity and progression in ovarian cancer by functioning as a miR\u2010302e sponge to positively regulate VEGFA"],"prefix":"10.1111","volume":"23","author":[{"given":"Li\u2010Li","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gynecologic Oncology Research Office The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institute in Guangdong Province Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Hong","family":"Zong","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology Research Office The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institute in Guangdong Province Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gynecology The First Affiliated Hospital of China Medical University Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Gynecology The First Affiliated Hospital of China Medical University Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology Research Office The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institute in Guangdong Province Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3226-8127","authenticated-orcid":false,"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology Research Office The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institute in Guangdong Province Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-1931"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-016-1837-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-017-1581-x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2016.05.026"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12186"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.1.7678559"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.001"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.035667.112"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw027"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.09.011"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19930"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101254"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.12.097"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.08.012"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990531)81:5<682::AID-IJC2>3.0.CO;2-B"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i9.1950"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000073128.16098.31"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1998.23"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.04.003"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048917"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2013.06.004"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-477"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2009.11.015"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11993"},{"key":"e_1_2_11_26_1","first-page":"1566","article-title":"Circular RNA circ\u2010ABCB10 promotes breast cancer proliferation and progression through sponging miR\u20101271","volume":"7","author":"Liang HF","year":"2017","journal-title":"Am J Cancer Res"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2016.11.004"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.11.023"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2015.11.034"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369664"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/15680096113139990074"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2095-6"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000022200.71892.9F"},{"key":"e_1_2_11_34_1","first-page":"2131","article-title":"Downregulation of VEGFA inhibits proliferation, promotes apoptosis, and suppresses migration and invasion of renal clear cell carcinoma","volume":"9","author":"Zeng FC","year":"2016","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-014-9538-9"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1053"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-5290-9"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2015.02.030"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T16:53:56Z","timestamp":1694019236000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,22]]},"references-count":37,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/jcmm.14736"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14736","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,22]]},"assertion":[{"value":"2019-03-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}