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Emerging evidence indicates that microRNAs (miRNAs) play a pivotal role in a variety of diseases and have been identified as critical regulators of the innate immune response. Recent miRNome profile analysis revealed an altered expression level of miR\u2010148a in cows with endometritis. Therefore, the present study aims to investigate the regulatory role of miR\u2010148a in the innate immune response involved in endometritis and estimate its potential therapeutic value. Here, we found that miR\u2010148a expression in lipopolysaccharide (LPS)\u2010stimulated endometrial epithelial cells was significantly decreased\n                    <jats:bold>.<\/jats:bold>\n                    Our results also showed that overexpression of miR\u2010148a using agomiR markedly reduced the production of pro\u2010inflammatory cytokines, such as IL\u20101\u03b2 and TNF\u2010\u03b1. Moreover, overexpression of miR\u2010148a also suppressed NF\u2010\u03baB p65 activation by targeting the TLR4\u2010mediated pathway. Subsequently, we further verified that miR\u2010148a repressed TLR4 expression by binding to the 3\u2032\u2010UTR of TLR4 mRNA. Additionally, an experimental mouse endometritis model was employed to evaluate the therapeutic value of miR\u2010148a. In vivo studies suggested that up\u2010regulation of miR\u2010148a alleviated the inflammatory conditions in the uterus as evidenced by H&amp;E staining, qPCR and Western blot assays, while inhibition of miR\u2010148a had inverse effects. Collectively, pharmacologic stabilization of miR\u2010148a represents a novel therapy for endometritis and other inflammation\u2010related diseases.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14744","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T08:43:48Z","timestamp":1574412228000},"page":"405-417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["miR\u2010148a suppresses inflammation in lipopolysaccharide\u2010induced endometritis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9248-0723","authenticated-orcid":false,"given":"Kangfeng","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Clinical Veterinary Medicine College of Veterinary Medicine Huazhong Agricultural University Wuhan China"},{"name":"Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and 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