{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:40:38Z","timestamp":1790005238828,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Key Science and Technology Research Project of Health and Family Planning Commission, Hebei Province","award":["ZD20130219"],"award-info":[{"award-number":["ZD20130219"]}]},{"name":"Hebei Provinical Administration of Traditional Chinese Medicine","award":["2014143"],"award-info":[{"award-number":["2014143"]}]},{"name":"Foundation for Hepatitis Prevention and Control, Tianqing Liver Disease Research Fund","award":["TQGB20140203"],"award-info":[{"award-number":["TQGB20140203"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Primary biliary cholangitis (PBC) is an autoimmune disease characterized by chronic destruction of the bile ducts. A major unanswered question regarding the pathogenesis of PBC is the precise mechanisms of small bile duct injury. Emperipolesis is one of cell\u2010in\u2010cell structures that is a potential histological hallmark associated with chronic hepatitis B. This study aimed to clarify the pathogenesis and characteristics of emperipolesis in PBC liver injury. Sixty\u2010six PBC patients, diagnosed by liver biopsy combined with laboratory test, were divided into early\u2010stage PBC (stages I and II, n\u00a0=\u00a039) and late\u2010stage PBC (stages III and IV, n\u00a0=\u00a027). Emperipolesis was measured in liver sections stained with haematoxylin\u2010eosin. The expressions of CK19, CD3, CD4, CD8, CD20, Ki67 and apoptosis of BECs were evaluated by immunohistochemistry or immunofluorescence double labelling. Emperipolesis was observed in 62.1% of patients with PBC, and BECs were predominantly host cells. The number of infiltrating CD3\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells correlated with the advancement of emperipolesis (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a00.318,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a0.001;\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a00.060,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). The cell numbers of TUNEL\u2010positive BECs and double staining for CK19 and Ki67 showed a significant positive correlation with emperipolesis degree (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a00.236,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a0.001;\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a00.267,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a0.001). We conclude that emperipolesis mediated by CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells appears to be relevant to apoptosis of BEC and thus may aggravate the further injury of interlobular bile ducts.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14752","type":"journal-article","created":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T13:27:29Z","timestamp":1576675649000},"page":"1268-1275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Emperipolesis mediated by CD8\n                    <sup>+<\/sup>\n                    T cells correlates with biliary epithelia cell injury in primary biliary cholangitis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6674-4721","authenticated-orcid":false,"given":"Su\u2010xian","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010cong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0563-9925","authenticated-orcid":false,"given":"Na","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010de","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology Institution The Fifth Medical Center, General Hospital of PLA Beijing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology Institution The Fifth Medical Center, General Hospital of PLA Beijing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010na","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology Institution The Fifth Medical Center, General Hospital of PLA Beijing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010guo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong\u2010qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue\u2010min","family":"Nan","sequence":"additional","affiliation":[{"name":"Department of Traditional and Western Medical Hepatology The Third Hospital of Hebei Medical University Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010min","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology Institution The Fifth Medical Center, General Hospital of PLA Beijing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30145"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0106-9543.2002.01595.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-010-9280-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)00075-1"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Viral and autoimmune hepatitis. Morphologic and pathogenetic aspects of cell damage in hepatitis with potential chronicity","volume":"132","author":"Dienes HP","year":"1989","journal-title":"Veroff Pathol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-014-8432-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524015666151026105411"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.09.015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:28:2:083"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.35067"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2003.09.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.36161"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25511"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2003.11.002"},{"issue":"7","key":"e_1_2_9_16_1","first-page":"1213","article-title":"Portal hepatic lymphadenopathy involved by Rosai\u2010Dorfman disease\u2010like changes in a patient with hepatocellular carcinoma","volume":"109","author":"Takagi H","year":"2012","journal-title":"Nihon Shokakibyo Gakkai Zasshi"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/IJPM.IJPM_409_18"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjq002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09588"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524015666151026102328"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802702105"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14752","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:01:15Z","timestamp":1788447675000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,18]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/jcmm.14752"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14752","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,18]]},"assertion":[{"value":"2019-07-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}