{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:27:08Z","timestamp":1788449228274,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>TIM\u20104 plays an important role in ischaemia\u2010reperfusion injury of liver and kidney; however, the effects of TIM\u20104 on cerebral ischaemia\u2010reperfusion injury (IRI) are unknown. The purpose of the present study was to investigate the potential role of TIM\u20104 in experimental brain ischaemia\u2010reperfusion injury. In this study, cerebral ischaemia reperfusion was induced by transient middle cerebral artery occlusion (MCAO) for 1\u00a0hour in C57\/BL6 mice. The TIM\u20104 expression was detected in vivo or vitro by real\u2010time quantitative polymerase chain reaction, Western blot and flow cytometric analysis. In vivo, the administration of anti\u2010TIM\u20104 antibodies significantly suppressed apoptosis, inhibited inflammatory cells and enhanced anti\u2010inflammatory responses. In vitro, activated microglia exhibited reduced cellular proliferation and induced IRI injury when co\u2010cultured with neurons; these effects were inhibited by anti\u2010TIM\u20104 antibody treatment. Similarly, microglia transfected with TIM\u20104 siRNA and stimulated by LPS\u00a0+\u00a0IFN\u2010\u03b3 alleviated the TIM\u20104\u2010mediated damage to neurons. Collectively, our data indicate that the inhibition of TIM\u20104 can improve the inflammatory response and exerts a protective effect in cerebral ischaemia\u2010reperfusion injury.<\/jats:p>","DOI":"10.1111\/jcmm.14754","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T09:01:36Z","timestamp":1574845296000},"page":"1276-1285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of TIM\u20104 protects against cerebral ischaemia\u2010reperfusion injury"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8726-0547","authenticated-orcid":false,"given":"Lifang","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Neurology The Seventh People's Hospital of Shenzhen Shenzhen China"},{"name":"Department of Neurology Puren Hospital Affiliated to Wuhan University of Science and Technology Wuhan 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