{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:19:54Z","timestamp":1790018394078,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572155, 81873993"],"award-info":[{"award-number":["81572155, 81873993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Type 2 diabetes mellitus (T2DM) is the most common diabetes and has numerous complications. Recent studies demonstrated that T2DM compromises bone fracture healing in which miR\u2010222 might be involved. Furthermore, tissue inhibitor of metalloproteinase 3 (TIMP\u20103) that is the target of miR\u2010222 accelerates fracture healing. Therefore, we assume that miR\u2010222 could inhibit TIMP\u20103 expression. Eight\u2010week\u2010old rats were operated femoral fracture or sham, following the injection of streptozotocin (STZ) to induce diabetes one week later in fractured rats, and then, new generated tissues were collected for measuring the expression of miR\u2010222 and TIMP\u20103. Rat mesenchymal stem cells (MSCs) were isolated and treated with miR\u2010222 mimic or inhibitor to analyse osteogenic differentiation. MiR\u2010222 was increased in fractured rats and further induced in diabetic rats. In contrast, TIMP\u20103 was reduced in fractured and further down\u2010regulated in diabetic rats. Luciferase report assay indicated miR\u2010222 directly binds and mediated TIMP\u20103. Furthermore, osteogenic differentiation was suppressed by miR\u2010222 mimic and promoted by miR\u2010222 inhibitor. miR\u2010222 is a key regulator that is promoted in STZ\u2010induced diabetic rats, and it binds to TIMP3 to reduce TIMP\u20103 expression and suppressed MSCs\u2019 differentiation.<\/jats:p>","DOI":"10.1111\/jcmm.14777","type":"journal-article","created":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T09:00:23Z","timestamp":1573030823000},"page":"686-694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Inhibition of microRNA\u2010222 up\u2010regulates TIMP3 to promotes osteogenic differentiation of MSCs from fracture rats with type 2 diabetes mellitus"],"prefix":"10.1111","volume":"24","author":[{"given":"Chenyi","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital Shanghai 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