{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:33:08Z","timestamp":1790512388867,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373202"],"award-info":[{"award-number":["81373202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mitochondrial fission and fusion are important for mitochondrial function, and dynamin 1\u2010like protein (DNM1L) is a key regulator of mitochondrial fission. We investigated the effect of mitochondrial fission on mitochondrial function and inflammation in fibroblast\u2010like synoviocytes (FLSs) during rheumatoid arthritis (RA). DNM1L expression was determined in synovial tissues (STs) from RA and non\u2010RA patients. FLSs were isolated from STs and treated with a DNM1L inhibitor (mdivi\u20101, mitochondrial division inhibitor 1) or transfected with\n                    <jats:italic>DNM1L<\/jats:italic>\n                    \u2010specific siRNA. Mitochondrial morphology, DNM1L expression, cell viability, mitochondrial membrane potential, reactive oxygen species (ROS), apoptosis, inflammatory cytokine expression and autophagy were examined. The impact of mdivi\u20101 treatment on development and severity of collagen\u2010induced arthritis (CIA) was determined in mice. Up\u2010regulated DNM1L expression was associated with reduced mitochondrial length in STs from patients with RA and increased RA severity. Inhibition of DNM1L in FLSs triggered mitochondrial depolarization, mitochondrial elongation, decreased cell viability, production of ROS, IL\u20108 and COX\u20102, and increased apoptosis. DNM1L deficiency inhibited IL\u20101\u03b2\u2013mediated AKT\/IKK activation, NF\u2010\u03baBp65 nuclear translocation and LC3B\u2010related autophagy, but enhanced NFKBIA expression. Treatment of CIA mice with mdivi\u20101 decreased disease severity by modulating inflammatory cytokine and ROS production. Our major results are that up\u2010regulated DNM1L and mitochondrial fission promoted survival, LC3B\u2010related autophagy and ROS production in FLSs, factors that lead to inflammation by regulating AKT\/IKK\/NFKBIA\/NF\u2010\u03baB signalling. Thus, inhibition of DNM1L may be a new strategy for treatment of RA.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.14837","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T23:45:34Z","timestamp":1574379934000},"page":"1516-1528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Inhibition of DNM1L and mitochondrial fission attenuates inflammatory response in fibroblast\u2010like synoviocytes of rheumatoid arthritis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2980-047X","authenticated-orcid":false,"given":"Xiaoyan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology The Second Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhufeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department 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