{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:44:21Z","timestamp":1773287061082,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 CA140654"],"award-info":[{"award-number":["R01 CA140654"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cyclin\u2010dependent kinase 7 (CDK7) is a protein kinase that plays a major role in transcription initiation. Yes\u2010associated protein (YAP) is a main effector of the Hippo\/YAP signalling pathway. Here, we investigated the role of CDK7 on YAP regulation in human malignant pleural mesothelioma (MPM). We found that in microarray samples of human MPM tissue, immunohistochemistry staining showed correlation between the expression level of CDK7 and YAP (n\u00a0=\u00a070, <jats:italic>r<\/jats:italic>\u00a0=\u00a0.513). In MPM cells, CDK7 expression level was significantly correlated with GTIIC reporter activity (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.886, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.019). Inhibition of CDK7 by siRNA decreased the YAP protein level and the GTIIC reporter activity in the MPM cell lines 211H, H290 and H2052. Degradation of the YAP protein was accelerated after CDK7 knockdown in 211H, H290 and H2052 cells. Inhibition of CDK7 reduced tumour cell migration and invasion, as well as tumorsphere formation ability. Restoration of the CDK7 gene rescued the YAP protein level and GTIIC reporter activity after siRNA knockdown in 211H and H2052 cells. Finally, we performed a co\u2010immunoprecipitation analysis using an anti\u2010YAP antibody and captured the CDK7 protein in 211H cells. Our results suggest that CDK7 inhibition reduces the YAP protein level by promoting its degradation and suppresses the migration and invasion of MPM cells. Cyclin\u2010dependent kinase 7 may be a promising therapeutic target for MPM.<\/jats:p>","DOI":"10.1111\/jcmm.14841","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T06:07:28Z","timestamp":1574402848000},"page":"1087-1098","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibition of cyclin\u2010dependent kinase 7 down\u2010regulates yes\u2010associated protein expression in mesothelioma cells"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5027-813X","authenticated-orcid":false,"given":"Jinbai","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Surgery Thoracic Oncology Laboratory Comprehensive Cancer Center University of California San Francisco CA USA"},{"name":"Department of Thoracic Surgery Beijing Chao\u2010Yang Hospital Affiliated with Capital Medical University Beijing 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