{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:46:08Z","timestamp":1791654368642,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M600826"],"award-info":[{"award-number":["2016M600826"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560267"],"award-info":[{"award-number":["81560267"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960302"],"award-info":[{"award-number":["81960302"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Excessive neutrophil extracellular trap (NET) formation may contribute to polymyositis (PM)\u2010associated interstitial lung diseases (ILD), but the underlying mechanism is not fully revealed. In this study, we found that NET accelerated the progression of ILD and promoted pulmonary fibrosis (PF) in vivo. miR\u20107 expression was down\u2010regulated in lung tissue of PM group than control group, and NETs further decreased miR\u20107 expression. TLR9 and Smad2 were up\u2010regulated in lung tissue of PM group than control group, and NETs further increased TLR9 and Smad2 expressions. In vitro experiments showed that PMA\u2010treated NETs accelerated the proliferation of LF and their differentiation into myofibroblast (MF), whereas DNase I decreased the promotion effect of NETs. Neutrophil extracellular trap components myeloperoxidase (MPO) and histone 3 also promoted the proliferation and differentiation of LF. In addition, we demonstrated that TLR9 involved in the regulation of NETs on LF proliferation and differentiation, and confirmed the interaction between miR\u20107 and Smad2 in LF. Finally, miR\u20107\u2010Smad2 pathway was confirmed to be involved in the regulation of TLR9 on LF proliferation and differentiation. Therefore, NETs promote PM\u2010related ILD, and TLR9\u2010miR\u20107\u2010Smad2 signalling pathway is involved in the proliferation of LFs and their differentiation into MFs.<\/jats:p>","DOI":"10.1111\/jcmm.14858","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T10:52:28Z","timestamp":1575975148000},"page":"1658-1669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Neutrophil extracellular traps activate lung fibroblast to induce polymyositis\u2010related interstitial lung diseases via TLR9\u2010miR\u20107\u2010Smad2 pathway"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1565-1431","authenticated-orcid":false,"given":"Sigong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Lanzhou University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junnian","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pneumology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Lanzhou University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyue","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1618-6765","authenticated-orcid":false,"given":"Haili","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.02.007"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kev192"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2377-11-143"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30513"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23073-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0121TR"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.02.004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4359"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-15-51"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0193PS"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2015.03.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00311"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-017-3799-y"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12319"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MIB.0000436276.19755.c1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.201201-002AW"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.12.011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001510"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1591"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29153"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201445286"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2014.1764"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1459"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-07-0519"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2017.11.005"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-014-0155-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2015-0205OC"},{"issue":"77","key":"e_1_2_8_30_1","article-title":"Isolation purification and labeling of mouse bone marrow neutrophils for functional studies and adoptive transfer experiments","volume":"10","author":"Swamydas M","year":"2013","journal-title":"J Vis Exp"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1708247114"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.201608"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032366"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000460293"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix475"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27841"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00060"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2016.00054"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-017-9404-4"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.116.239137"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00481.2016"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26961"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.104"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14858","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14858","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:00:57Z","timestamp":1788447657000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,10]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/jcmm.14858"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14858","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,10]]},"assertion":[{"value":"2018-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}