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The results showed that pre\u2010treatment with APP\u2010AW, S1, S2 and S3 each at the concentration of 50\u00a0\u03bcg\/mL for 48\u00a0hours was able to prevent cytotoxicity induced by 1\u00a0\u03bcmol\/L dexamethasone (Dex) in MC3T3\u2010E1 cells via inhibition of apoptosis, which is in line with the findings in flow cytometry analysis. Meanwhile, the decreased ALP activity, collagen content, mineralization, BMP2, Runx2, OSX and OCN protein expression in DEX\u2010treated MC3T3\u2010E1 cells were reversed by the addition of APP\u2010AW, S1, S2 and S3. Moreover, APP\u2010AW, S1, S2 and S3 rescued DEX\u2010induced increase of Bax, cytochrome c and caspase\u20103 and decrease of Bcl\u20102, Wnt3, \u03b2\u2010catenin and c\u2010Myc protein expression in MC3T3\u2010E1 cells. Our findings suggest that pre\u2010treatment with APP\u2010AW, S1, S2 and S3 could significantly protect MC3T3\u2010E1 cells against Dex\u2010induced cell injury via inhibiting apoptosis and activating Wnt\/\u03b2\u2010Catenin signalling pathway, thus application of these polysaccharides may be a promising alternative strategy for steroid\u2010induced avascular necrosis of the femoral head (SANFH) therapy.<\/jats:p>","DOI":"10.1111\/jcmm.14868","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T02:59:25Z","timestamp":1579489165000},"page":"2169-2177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Protective effect of <i>Agrimonia pilosa<\/i> polysaccharides on dexamethasone\u2010treated MC3T3\u2010E1 cells via Wnt\/\u03b2\u2010Catenin pathway"],"prefix":"10.1111","volume":"24","author":[{"given":"Wei","family":"Huang","sequence":"first","affiliation":[{"name":"Department of 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