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Amurensin H is a resveratrol dimer with anti\u2010inflammatory and anti\u2010apoptotic effects, while its effects on TLR\u20104 signals to inhibit osteoarthritis are still unclear. In the present study, treatment with amurensin H for 2\u00a0weeks in monosodium iodoacetate\u2010induced mice significantly slows down cartilage degeneration and inflammation using macroscopic evaluation, haematoxylin and eosin (HE) staining and micro\u2010magnetic resonance imaging. In IL\u20101\u03b2\u2010stimulated rat chondrocytes, amurensin H suppresses the production of inflammatory mediators including nitric oxide, IL\u20106, IL\u201017, PGE2 and TNF\u2010\u03b1 using Greiss and ELISA assay. Amurensin H inhibits matrix degradation via decreasing levels of MMP\u20109 and MMP\u201013 using Western blot assay, promotes synthesis of type II collagen and glycosaminoglycan using immunostaining and safranin O staining, respectively. Amurensin H inhibits intracellular and mitochondrial reactive oxygen species (ROS) generation, and mitochondrial membrane depolarization using DCFH\u2010DA, MitoSOX Red and JC\u20101 assay as well. IL\u20101\u03b2 stimulates TLR4 activation and Syk phosphorylation in chondrocytes, while amurensin H inhibits TLR4\/Syk signals and downstream p65 phosphorylation and translocation in a time and dose\u2010dependent manner. Together, these results suggest that amurensin H exerts chondroprotective effects by attenuating oxidative stress, inflammation and matrix degradation via the TLR4\/Syk\/NF\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1111\/jcmm.14893","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T02:13:22Z","timestamp":1577326402000},"page":"1958-1968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Chondroprotective and anti\u2010inflammatory effects of amurensin H by regulating TLR4\/Syk\/NF\u2010\u03baB signals"],"prefix":"10.1111","volume":"24","author":[{"given":"Pei","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing 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