{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:10:57Z","timestamp":1788491457714,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,19]],"date-time":"2020-01-19T00:00:00Z","timestamp":1579392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["70112142"],"award-info":[{"award-number":["70112142"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["70112717"],"award-info":[{"award-number":["70112717"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chronic myeloid leukaemia (CML) is a clonal myeloproliferative stem cell disorder characterized by the constitutively active BCR\u2010ABL tyrosine kinase. The LIM and SH3 domain protein 1 (LASP1) has recently been identified as a novel BCR\u2010ABL substrate and is associated with proliferation, migration, tumorigenesis and chemoresistance in several cancers. Furthermore, LASP1 was shown to bind to the chemokine receptor 4 (CXCR4), thought to be involved in mechanisms of relapse. In order to identify potential LASP1\u2010mediated pathways and related factors that may help to further eradicate minimal residual disease (MRD), the effect of LASP1 on processes involved in progression and maintenance of CML was investigated. The present data indicate that not only overexpression of CXCR4, but also knockout of LASP1 contributes to proliferation, reduced apoptosis and migration as well as increased adhesive potential of K562 CML cells. Furthermore, LASP1 depletion in K562 CML cells leads to decreased cytokine release and reduced NK cell\u2010mediated cytotoxicity towards CML cells. Taken together, these results indicate that in CML, reduced levels of LASP1 alone and in combination with high CXCR4 expression may contribute to TKI resistance.<\/jats:p>","DOI":"10.1111\/jcmm.14910","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T02:19:51Z","timestamp":1579486791000},"page":"2942-2955","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Knockout of LASP1 in CXCR4 expressing CML cells promotes cell persistence, proliferation and TKI resistance"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3633-9535","authenticated-orcid":false,"given":"Andreas B.","family":"Herrmann","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Experimentelle Biomedizin II Universit\u00e4tsklinikum W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha\u2010Lena","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Rudolf\u2010Virchow\u2010Zentrum f\u00fcr Experimentelle Biomedizin I Universit\u00e4tsklinikum W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin F.","family":"Orth","sequence":"additional","affiliation":[{"name":"Labor f\u00fcr P\u00e4diatrische Sarkombiologie Medizinische Fakult\u00e4t Pathologisches Institut LMU M\u00fcnchen M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg P.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Center for Molecular Biomedicine Institut f\u00fcr Molekulare Zellbiologie Universit\u00e4tsklinikum Jena Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma","family":"Zernecke","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Biomedizin II Universit\u00e4tsklinikum W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Hochhaus","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr H\u00e4matologie und internistische Onkologie Klinik f\u00fcr Innere Medizin II Universit\u00e4tsklinikum Jena Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Ernst","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr H\u00e4matologie und internistische Onkologie Klinik f\u00fcr Innere Medizin II Universit\u00e4tsklinikum Jena Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Butt","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Biomedizin II Universit\u00e4tsklinikum W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1476-8059","authenticated-orcid":false,"given":"Jochen J.","family":"Frietsch","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr H\u00e4matologie und internistische Onkologie Klinik f\u00fcr Innere Medizin II Universit\u00e4tsklinikum Jena Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899-017-0409-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1609324"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-01-617993"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-017-2295-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70233-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-02-483750"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V128.22.787.787"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-0042"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2072"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3083"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317705757"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00391"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts059"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.11.002"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010050"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2015.166"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-210732"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-07-511907"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni179"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.10.010"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.08.008"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404549"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-2040"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.04.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.119"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-13-0410"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.021"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.5"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-018-2604-x"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11720"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90247.2008"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129219"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1489"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605685"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2012.09.032"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200505018"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-08-265892"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000389"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.239"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899-010-0043-0"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.1.319"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(05)70188-X"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2011.242"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.14910","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14910","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.14910","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.14910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T10:20:51Z","timestamp":1767176451000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.14910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,19]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1111\/jcmm.14910"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.14910","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,19]]},"assertion":[{"value":"2019-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}