{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:14:24Z","timestamp":1788952464233,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570267"],"award-info":[{"award-number":["81570267"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010083","name":"Hunan Provincial Innovation Foundation for Postgraduate","doi-asserted-by":"publisher","award":["CX2018B068"],"award-info":[{"award-number":["CX2018B068"]}],"id":[{"id":"10.13039\/501100010083","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The specific mechanism of pulmonary arterial hypertension (PAH) remains elusive. The present study aimed to explore the underlying mechanism of PAH through the identity of novel biomarkers for PAH using metabolomics approach. Serum samples from 40 patients with idiopathic PAH (IPAH), 20 patients with congenital heart disease\u2010associated PAH (CHD\u2010PAH) and 20 healthy controls were collected and analysed by ultra\u2010high\u2010performance liquid chromatography coupled with high\u2010resolution mass spectrometry (UPLC\u2010HRMS). Orthogonal partial least square\u2010discriminate analysis (OPLS\u2010DA) was applied to screen potential biomarkers. These results were validated in monocrotaline (MCT)\u2010induced PAH rat model. The OPLS\u2010DA model was successful in screening distinct metabolite signatures which distinguished IPAH and CHD\u2010PAH patients from healthy controls, respectively (26 and 15 metabolites). Unbiased analysis from OPLS\u2010DA identified 31 metabolites from PAH patients which were differentially regulated compared to the healthy controls. Our analysis showed dysregulation of the different metabolic pathways, including lipid metabolism, glucose metabolism, amino acid metabolism and phospholipid metabolism pathways in PAH patients compared to their healthy counterpart. Among these metabolites from dysregulated metabolic pathways, a panel of metabolites from lipid metabolism and fatty acid oxidation (lysophosphatidylcholine, phosphatidylcholine, perillic acid, palmitoleic acid, N\u2010acetylcholine\u2010<jats:sc>d<\/jats:sc>\u2010sphingomyelin, oleic acid, palmitic acid and 2\u2010Octenoylcarnitine metabolites) were found to have a close association with PAH. The results from the analysis of both real\u2010time quantitative PCR and Western blot showed that expression of LDHA, CD36, FASN, PDK1 GLUT1 and CPT\u20101 in right heart\/lung were significantly up\u2010regulated in MCT group than the control group.<\/jats:p>","DOI":"10.1111\/jcmm.14937","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:04:53Z","timestamp":1579219493000},"page":"2484-2496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Metabolomics reveals metabolite changes of patients with pulmonary arterial hypertension in China"],"prefix":"10.1111","volume":"24","author":[{"given":"Chenyang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Xiangya Hospital Central South University Changsha China"},{"name":"Department of Cardiovascular Medicine The Third Xiangya Hospital Central South University Changsha 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