{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T06:15:47Z","timestamp":1791699347706,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572116"],"award-info":[{"award-number":["81572116"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772361"],"award-info":[{"award-number":["81772361"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572158"],"award-info":[{"award-number":["81572158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aseptic loosening caused by wear particles is a common complication after total hip arthroplasty. We investigated the effect of the quercetin on wear particle\u2010mediated macrophage polarization, inflammatory response and osteolysis. In vitro, we verified that Ti particles promoted the differentiation of RAW264.7 cells into M1 macrophages through p\u201038\u03b1\/\u03b2 signalling pathway by using flow cytometry, immunofluorescence assay and small interfering p\u201038\u03b1\/\u03b2 RNA. We used enzyme\u2010linked immunosorbent assays to confirm that the protein expression of M1 macrophages increased in the presence of Ti particles and that these pro\u2010inflammatory factors further regulated the imbalance of OPG\/RANKL and promoted the differentiation of osteoclasts. However, this could be suppressed, and the protein expression of M2 macrophages was increased by the presence of the quercetin. In vivo, we revealed similar results in the mouse skull by \u03bc\u2010CT, H&amp;E staining, immunohistochemistry and immunofluorescence assay. We obtained samples from patients with osteolytic tissue. Immunofluorescence analysis indicated that most of the macrophages surrounding the wear particles were M1 macrophages and that pro\u2010inflammatory factors were released. Titanium particle\u2010mediated M1 macrophage polarization, which caused the release of pro\u2010inflammatory factors through the p\u201038\u03b1\/\u03b2 signalling pathway, regulated OPG\/RANKL balance. Macrophage polarization is expected to become a new clinical drug therapeutic target.<\/jats:p>","DOI":"10.1111\/jcmm.14995","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T08:43:30Z","timestamp":1581583410000},"page":"3203-3216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Quercetin inhibits macrophage polarization through the p\u201038\u03b1\/\u03b2 signalling pathway and regulates OPG\/RANKL balance in a mouse skull model"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-7807","authenticated-orcid":false,"given":"Yu\u2010Wei","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3388-6728","authenticated-orcid":false,"given":"Kai","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"},{"name":"Department of Orthopedic Surgery Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Liang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010An","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Fang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Yun","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine Shangai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of 2nd Dental Center Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; 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