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Recent evidence has identified cross\u2010talk between transforming growth factor beta (TGFB) and the WNT\/\u03b2\u2010catenin pathway to induce fibrosis and angiogenesis. Limited evidence exists describing the role of non\u2010canonical WNT signalling in peritoneal membrane injury. Non\u2010canonical WNT5A is suggested to have different effects depending on the receptor environment. WNT5A has been implicated in antagonizing canonical WNT\/\u03b2\u2010catenin signalling in the presence of receptor tyrosine kinase\u2010like orphan receptor (Ror2). We co\u2010expressed TGFB and WNT5A using adenovirus and examined its role in the development of peritoneal fibrosis and angiogenesis. Treatment of mouse peritoneum with AdWNT5A decreased the submesothelial thickening and angiogenesis induced by AdTGFB. WNT5A appeared to block WNT\/\u03b2\u2010catenin signalling by inhibiting phosphorylation of glycogen synthase kinase 3 beta (GSK3B) and reducing levels of total \u03b2\u2010catenin and target proteins. To examine the function of Ror2, we silenced Ror2 in a human mesothelial cell line. We treated cells with AdWNT5A and observed a significant increase in fibronectin compared with AdWNT5A alone. We also analysed fibronectin and vascular endothelial growth factor (VEGF) in a TGFB model of mesothelial cell injury. Both fibronectin and VEGF were significantly increased in response to Ror2 silencing when cells were exposed to TGFB. Our results suggest that WNT5A inhibits peritoneal injury and this is associated with a decrease in WNT\/\u03b2\u2010catenin signalling. In human mesothelial cells, Ror2 is involved in regulating levels of fibronectin and VEGF.<\/jats:p>","DOI":"10.1111\/jcmm.15034","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T09:07:16Z","timestamp":1581584836000},"page":"3481-3491","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The role of WNT5A and Ror2 in peritoneal membrane injury"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9707-9936","authenticated-orcid":false,"given":"Manreet","family":"Padwal","sequence":"first","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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