{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:08:56Z","timestamp":1787162936336,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T00:00:00Z","timestamp":1581984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Funding of China","award":["81601983"],"award-info":[{"award-number":["81601983"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>As a chronic musculoskeletal degeneration disease, intervertebral disc degeneration (IVDD) has been identified as a crucial cause for low back pain. This condition has a prevalence of 80% among adults without effective preventative therapy. Procyanidin B3 (Pro\u2010B3) is a procyanidin dimer, which is widely present in the human diet and has multiple functions, such as preventing inflammation. But the inhibiting effect of Pro\u2010B3 in IVDD development is still no known. Thus, our study aimed to demonstrate the therapeutical effect of Pro\u2010B3 in IVDD and explain the underlying mechanism. In vitro studies, human nucleus pulposus (NP) cells were isolated and exposed in lipopolysaccharide (LPS) to simulate IVDD development. Pro\u2010B3 pre\u2010treatment inhibited LPS\u2010induced production of inflammation correlated factors such as tumour necrosis factor \u03b1 (TNF\u2010\u03b1), interleukin\u20106 (IL\u20106), prostaglandin E2 (PGE2) and Nitric oxide (NO). On the other hand, LPS\u2010medicated extracellular matrix (ECM) breakdown was blocked in Pro\u2010B3 treated NP cells. Additionally, Pro\u2010B3 treatment blocked the activation of NF\u2010\u03baB\/toll\u2010like receptor 4 pathway in LPS\u2010exposed NP cells. Mechanistically, Pro\u2010B3 could occupy MD\u20102's hydrophobic pocket exhibiting high affinity for LPS to intervene LPS\/TLR4\/MD\u20102 complex formation. In vivo, Pro\u2010B3 treatment prevented the loss of gelatin NP cells and structural damage of annulus fibrosus in rat IVDD model. In brief, Pro\u2010B3 is considered to be a treatment agent for IVDD.<\/jats:p>","DOI":"10.1111\/jcmm.15074","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T08:55:15Z","timestamp":1582016115000},"page":"3701-3711","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Procyanidin B3 alleviates intervertebral disc degeneration via interaction with the TLR4\/MD\u20102 complex"],"prefix":"10.1111","volume":"24","author":[{"given":"Ping","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Rehabilitation The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Jiao Tong University Affiliated Shanghai Sixth People\u2019s Hospital Shanghai 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