{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:11:31Z","timestamp":1790946691947,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"the Natural Science Fund of China","award":["81472105"],"award-info":[{"award-number":["81472105"]}]},{"name":"the Natural Science Fund of China","award":["81874008"],"award-info":[{"award-number":["81874008"]}]},{"name":"the Soochow health program of talent training","award":["GSWS2019010"],"award-info":[{"award-number":["GSWS2019010"]}]},{"name":"the second affiliated hospital of Soochow university preponderant clinic discipline project funding","award":["XKQ2015003"],"award-info":[{"award-number":["XKQ2015003"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The increase in bone resorption and\/or the inhibition of bone regeneration caused by wear particles are the main causes of periprosthetic osteolysis. The SOST gene and Sclerostin, a protein synthesized by the SOST gene, are the characteristic marker of osteocytes and regulate bone formation and resorption. We aimed to verify whether the SOST gene was involved in osteolysis induced by titanium (Ti) particles and to investigate the effects of SOST reduction on osteolysis. The results showed osteolysis on the skull surface with an increase of sclerostin levels after treated with Ti particles. Similarly, sclerostin expression in MLO\u2010Y4 osteocytes increased when treated with Ti particles in vitro. After reduction of SOST, local bone mineral density and bone volume increased, while number of lytic pores on the skull surface decreased and the erodibility of the skull surface was compensated. Histological analyses revealed that SOST reduction increased significantly alkaline phosphatase\u2010 (ALP) and osterix\u2010positive expression on the skull surface which promoted bone formation. ALP activity and mineralization of MC3T3\u2010E1 cells also increased in vitro when SOST was silenced, even if treated with Ti particles. In addition, Ti particles decreased \u03b2\u2010catenin expression with an increase in sclerostin levels, in vivo and in vitro. Inversely, reduction of SOST expression increased \u03b2\u2010catenin expression. In summary, our results suggested that reduction of SOST gene can activate the Wnt\/\u03b2\u2010catenin signalling pathway, promoting bone formation and compensated for bone loss induced by Ti particles. Thus, this study provided new perspectives in understanding the mechanisms of periprosthetic osteolysis.<\/jats:p>","DOI":"10.1111\/jcmm.15084","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T08:42:13Z","timestamp":1583397733000},"page":"4233-4244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Reduction of SOST gene promotes bone formation through the Wnt\/\u03b2\u2010catenin signalling pathway and compensates particle\u2010induced osteolysis"],"prefix":"10.1111","volume":"24","author":[{"given":"Zai Hang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Yu","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Soochow University Suzhou 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