{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T21:35:03Z","timestamp":1788816903018,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874242"],"award-info":[{"award-number":["81874242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800979"],"award-info":[{"award-number":["31800979"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772917"],"award-info":[{"award-number":["81772917"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472852"],"award-info":[{"award-number":["81472852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Malignant melanoma is one of the most invasive tumours. However, effective therapeutic strategies are limited, and overall survival rates remain low. By utilizing transcriptomic profiling, tissue array and molecular biology, we revealed that two key ubiquitin\u2010specific proteases (USPs), ubiquitin\u2010specific peptidase10 (USP10) and ubiquitin\u2010specific peptidase10 (USP13), were significantly elevated in melanoma at the mRNA and protein levels. Spautin\u20101 has been reported as a USP10 and USP13 antagonist, and we demonstrated that spautin\u20101 has potent anti\u2010tumour effects as reflected by MTS and the colony formation assays in various melanoma cell lines without cytotoxic effects in HaCaT and JB6 cell lines. Mechanistically, we identified apoptosis and ROS\u2010mediated DNA damage as critical mechanisms underlying the spautin\u20101\u2010mediated anti\u2010tumour effect by utilizing transcriptomics, qRT\u2010PCR validation, flow cytometry, Western blotting and immunofluorescence staining. Importantly, by screening spautin\u20101 with targeted or chemotherapeutic drugs, we showed that spautin\u20101 exhibited synergy with cisplatin in the treatment of melanoma. Pre\u2010clinically, we demonstrated that spautin\u20101 significantly attenuated tumour growth in a cell line\u2010derived xenograft mouse model, and its anti\u2010tumour effect was further enhanced by cotreatment with cisplatin. Taken together, our study revealed a novel molecular mechanism of spautin\u20101 effecting in melanoma and identified a potential therapeutic strategy in treatment of melanoma patients.<\/jats:p>","DOI":"10.1111\/jcmm.15093","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T11:46:06Z","timestamp":1583322366000},"page":"4324-4340","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Potent USP10\/13 antagonist spautin\u20101 suppresses melanoma growth via ROS\u2010mediated DNA damage and exhibits synergy with cisplatin"],"prefix":"10.1111","volume":"24","author":[{"given":"Jia","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JiangLing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Molecular Biology Research Center and Center for Medical Genetics Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Plastic and Cosmetic Surgery Xiangya Hospital Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Xiangya Clinical Research Center for Cancer Immunotherapy Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Xiangya Clinical Research Center for Cancer Immunotherapy Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9317-774X","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Center and Center for Medical Genetics Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Xiangya Clinical Research Center for Cancer Immunotherapy Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Xiangya Clinical Research Center for Cancer Immunotherapy Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9976-2985","authenticated-orcid":false,"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xiangya Hospital Central South University Changsha China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Changsha China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Changsha China"},{"name":"Xiangya Clinical Research Center for Cancer Immunotherapy Central South University Changsha China"},{"name":"Research Center of Molecular Metabolomics Xiangya Hospital Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2010-0190"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt510"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2217\/mmt-2017-0026"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05661"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60802-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.11.012"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.10.042"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030288"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3321"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.443"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.032"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.08.037"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.07.025"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.07.046"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2004.01.004"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2015.07.008"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0810-7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-1947"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.428"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2017.11.019"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80118-3"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1725"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612811319220013"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02693-9"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1565707"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2313"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206034"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1112484"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401565"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-1273-5"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-0989"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0909-x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.101084"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0897-x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4002"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-017-0193-7"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2288-8_30"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.109391"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(03)00238-6"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1390636"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00110-5"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.09.009"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.384"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-015-1633-3"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2013.53.1590"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2019.102925"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.cad.0000176506.71304.ca"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-014-0277-5"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09627"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1210093"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.12.009"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-065201"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.01.009"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.15093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:00:09Z","timestamp":1770966009000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,4]]},"references-count":53,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1111\/jcmm.15093"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15093","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,4]]},"assertion":[{"value":"2019-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}