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Extracellular vesicles (EVs) carry a specific composition of proteins, lipids and nucleotides have been considered as essential mediators of cell\u2010cell communication. Their potential impact for crosstalk between tissues responsible for aqueous humour production and out\u2010flow is largely unknown. The study objective was to investigate the effects of EVs derived from non\u2010pigmented ciliary epithelium (NPCE) primary cells on the expression of Wnt proteins in a human primary trabecular meshwork (TM) cells and define the mechanism underlying exosome\u2010mediated regulation that signalling pathway. Consistent with the results in TM cell line, EVs released by both primary NPCE cells and NPCE cell line showed diminished pGSK3\u03b2 phosphorylation and decreased cytosolic levels of \u03b2\u2010catenin in primary TM cells. At the molecular level, we showed that NPCE exosome treatment downregulated the expression of positive GSK\u03b2 regulator\u2010AKT protein but increased the levels of GSK\u03b2 negative regulator\u2010PP2A protein in TM cells. NPCE exosome protein analysis revealed 584 miRNAs and 182 proteins involved in the regulation of TM cellular processes, including WNT\/\u03b2\u2010catenin signalling pathway, cell adhesion and extracellular matrix deposition. We found that negative modulator of Wnt signalling miR\u201029b was abundant in NPCE exosomal samples and treatment of TM cells with NPCE EVs significantly decreased COL3A1 expression. Suggesting that miR\u201029b can be responsible for decreased levels of WNT\/\u03b2\u2010catenin pathway. Overall, this study highlights a potential role of EVs derived from NPCE cells in modulating ECM proteins and TM canonical Wnt signalling.<\/jats:p>","DOI":"10.1111\/jcmm.15129","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T16:52:06Z","timestamp":1584118326000},"page":"4646-4658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Extracellular vesicle\u2010mediated crosstalk between NPCE cells and TM cells result in modulation of Wnt signalling pathway and ECM remodelling"],"prefix":"10.1111","volume":"24","author":[{"given":"Natalie","family":"Lerner","sequence":"first","affiliation":[{"name":"Clinical Biochemistry and Pharmacology Department Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Schreiber\u2010Avissar","sequence":"additional","affiliation":[{"name":"Clinical 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