{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:37:22Z","timestamp":1787809042649,"version":"build-2784847793"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T00:00:00Z","timestamp":1584489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774165"],"award-info":[{"award-number":["81774165"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873206"],"award-info":[{"award-number":["81873206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2019A1515010865"],"award-info":[{"award-number":["2019A1515010865"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The pathogenesis of non\u2010alcoholic fatty liver disease (NAFLD) is still not fully understood, and currently, no effective pharmacotherapy is available. Nuclear receptors (NRs) are important biological participants in NAFLD that exhibit great therapeutic potential. Chaihu Shugan powder (CSP) is a traditional Chinese medicine (TCM) formula that has a wide therapeutic spectrum including NAFLD, but the effective components and functional mechanisms of CSP are unclear. We adopted a network pharmacology approach using multiple databases for Gene Ontology (GO) enrichment analysis and the molecular complex detection (MCODE) method for a protein\u2010protein interaction (PPI) analysis, and we used molecular docking method to screen the NR targets and determine the corresponding CSP components. The screening results were validated through a NAFLD rat model that was used to explain the possible relationship between CSP and NAFLD. Finally, we screened PPAR\u03b3, FXR, PPAR\u03b1, RAR\u03b1 and PPAR\u03b4 as target genes and\n                    <jats:italic>quercetin<\/jats:italic>\n                    ,\n                    <jats:italic>kaempferol<\/jats:italic>\n                    ,\n                    <jats:italic>naringenin<\/jats:italic>\n                    ,\n                    <jats:italic>isorhamnetin<\/jats:italic>\n                    and\n                    <jats:italic>nobiletin<\/jats:italic>\n                    as target compounds. The five components were detected through high\u2010performance liquid chromatography\u2010mass spectrometry (HPLC\u2010MS), the results of which aligned with the docking experiments of PPAR\u03b3, PPAR\u03b1 and PPAR\u03b4. After CSP intervention, the NAFLD rat model showed ameliorated effects in terms of bodyweight, hepatic histopathology, and serum and liver lipids, and the mRNA levels of PPAR\u03b3, FXR, PPAR\u03b1 and RAR\u03b1 were significantly changed. The results from this study indicate that CSP exhibits healing effects in an NAFLD model and that the network pharmacology approach to screening NR targets and determining the corresponding CSP components is a practical strategy for explaining the mechanism by which CSP ameliorates NAFLD.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15166","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T01:50:35Z","timestamp":1584582635000},"page":"5168-5184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["A network pharmacology\u2010based approach to explore the effects of Chaihu Shugan powder on a non\u2010alcoholic fatty liver rat model through nuclear receptors"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7063-0647","authenticated-orcid":false,"given":"Huan","family":"Nie","sequence":"first","affiliation":[{"name":"School of Traditional 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