{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T09:01:54Z","timestamp":1765270914807,"version":"3.46.0"},"reference-count":16,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"LiaoNing Revitalization Talents Program","award":["XLYC1808031"],"award-info":[{"award-number":["XLYC1808031"]}]},{"name":"International S&T Cooperation Program of China","award":["2013DFA11150"],"award-info":[{"award-number":["2013DFA11150"]}]},{"name":"Clinical Research Plan of Shanghai Hospital Development Center","award":["16CR3037A"],"award-info":[{"award-number":["16CR3037A"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Gallbladder carcinoma (GBC) is a vicious and invasive disease. The major challenge in the clinical treatment of GBC is the lack of a suitable prognosis method. Chemokine receptors such as CXCR3, CXCR4 and CXCR7 play vital roles in the process of tumour progression and metastasis. Their expression levels and distribution are proven to be indicative of the progression of GBC, but are hard to be decoded by conventional pathological methods, and therefore, not commonly used in the prognosis of GBC. In this study, we developed a computer\u2010aided image analysis method, which we used to quantitatively measure the expression levels of CXCR3, CXCR4 and CXCR7 in the nuclei and cytoplasm of glandular and interstitial cells from a cohort of 55 GBC patients. We found that CXCR3, CXCR4 and CXCR7 expressions are associated with the clinicopathological variables of GBC. Cytoplasmic CXCR3, nuclear CXCR7 and cytoplasmic CXCR7 were significant predictive factors of histology invasion, whereas cytoplasmic CXCR4 and nuclear CXCR4 were significantly correlated with T and N stage and were associated with the overall survival and disease\u2010free survival. These results suggest that the quantification and localisation of CXCR3, CXCR4 and CXCR7 expressions in different cell types should be considered using computer\u2010aided assessment to improve the accuracy of prognosis in GBC.<\/jats:p>","DOI":"10.1111\/jcmm.15219","type":"journal-article","created":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T19:18:08Z","timestamp":1591643888000},"page":"7670-7674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Computer\u2010aided assessment of the chemokine receptors CXCR3, CXCR4 and CXCR7 expression in gallbladder carcinoma"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1933-5981","authenticated-orcid":false,"given":"Xiao","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Pathology and Forensic Medicine Dalian Medical University Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of General Surgery School of Medicine Xinhua Hospital Shanghai JiaoTong University Shanghai China"},{"name":"Institute of Biliary Tract Diseases Research Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8297-8007","authenticated-orcid":false,"given":"Chan Way","family":"Ng","sequence":"additional","affiliation":[{"name":"Invitrocue Pte Ltd. Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuoyu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Radiology Sun Yat\u2010sen University Cancer Center Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Wu","sequence":"additional","affiliation":[{"name":"Invitrocue Pte Ltd. Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shili","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biliary Tract Diseases Research Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuren","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Urology the second Affiliated Hospital of Dalian Medical University Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biliary Tract Diseases Research Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Forensic Medicine Dalian Medical University Dalian 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