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In addition, after surgical treatment of gynaecological tumour, osteoporosis often occurs due to the withdrawal of oestrogen. The major characteristic of osteoporosis is the low bone mass with micro\u2010architectural deteriorated bone tissue. And the main cause is the overactivation of osteoclastogenesis, which is one of the most important therapeutic targets. Inflammation could induce the interaction of RANKL\/RANK, which is the promoter of osteoclastogenesis. Triptolide is derived from the traditional Chinese herb\n                    <jats:italic>lei gong teng<\/jats:italic>\n                    , presented multiple biological effects, including anti\u2010cancer, anti\u2010inflammation and immunosuppression. We hypothesized that triptolide could inhibits osteoclastogenesis by suppressing inflammation activation. In this study, we confirmed that triptolide could suppress RANKL\u2010induced osteoclastogenesis in bone marrow mononuclear cells (BMMCs) and RAW264.7 cells and inhibited the osteoclast bone resorption functions. PI3K\u2010AKT\u2010NFATc1 pathway is one of the most important downstream pathways of RANKL\u2010induced osteogenesis. The experiments in vitro indicated that triptolide suppresses the activation of PI3K\u2010AKT\u2010NFATc1 pathway and the target point located at the upstream of AKT because both NFATc1 overexpression and AKT phosphorylation could ameliorate the triptolide suppression effects. The expression of MDM2 was elevated, which demonstrated the MDM\u2010p53\u2010induced cell death might contribute to the osteoclastogenesis suppression. Ovariectomy\u2010induced bone loss and inflammation activation were also found to be ameliorated in the experiments in vivo. In summary, the new effect of anti\u2010cancer drug triptolide was demonstrated to be anti\u2010osteoclastogenesis, and we demonstrated triptolide might be a promising therapy for bone loss caused by tumour.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15229","type":"journal-article","created":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T22:34:52Z","timestamp":1588113292000},"page":"6149-6161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Triptolide prevents bone loss via suppressing osteoclastogenesis through inhibiting PI3K\u2010AKT\u2010NFATc1 pathway"],"prefix":"10.1111","volume":"24","author":[{"given":"Jin","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Orthopedics Shanghai Changhai Hospital Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai 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