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However, the role of CA9 in the molecular mechanisms of tongue squamous cell carcinoma (TSCC) pathogenesis remains unclear. CA9 expression was analysed using the TCGA database, and its influence on survival was performed using Kaplan\u2010Meier, LASSO and COX regression analyses. The correlation between CA9 and immune infiltration was investigated by CIBERSORT and ESTIMATE. Moreover, the relationship between CA9 expression and downstream molecular regulation pathways was analysed by GSEA, GO and WGCNA. CA9 expression correlated with clinical prognosis and tumour grade in TSCC. Moreover, CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour\u2010associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K\/AKR\/mTOR signalling and TGF\u2010beta signalling pathways. However, the follicular helper T cells, regulatory T cells, immune and stromal scores showed no significance between high and low CA9 expression groups. These findings suggested that CA9 plays a critical role of TSCC prognosis and tumour grade. CA9 expression significantly correlated with the regulation of cell differentiation, various oncogenes and cancer\u2010associated pathways.<\/jats:p>","DOI":"10.1111\/jcmm.15252","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T23:35:06Z","timestamp":1587080106000},"page":"5832-5841","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["CA9 transcriptional expression determines prognosis and tumour grade in tongue squamous cell carcinoma patients"],"prefix":"10.1111","volume":"24","author":[{"given":"Chenyu","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Guanghua School of Stomatology Hospital of Stomatology Sun Yat\u2010Sen University Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Stomatology Guangzhou 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