{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T09:02:17Z","timestamp":1765270937129,"version":"3.46.0"},"reference-count":15,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>FLT3<\/jats:italic>\n                      internal tandem duplication\u00a0(ITD) mutations are found in around 25% of all acute myeloid leukaemia\u00a0(AML) cases and is associated with shorter disease\u2010free and overall survival. Previous reports have shown that\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD induces a specific phenotype in leukemic blasts, which is characterized by high levels of CD33 and CD123, and that expression of\u00a0CD33 and CD123 is directly influenced by the DNA\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD\/wild\u2010type\u00a0\n                      <jats:italic>FLT3<\/jats:italic>\n                      allelic ratio (AR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 42\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD and 104\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD\u2013negative AML patients were analysed. Immunophenotyping data were used to calculate\u00a0antigen expression levels as\u00a0the ratio between the geometric mean fluorescence intensities (MFIs) of leukemic blasts and MFIs of negative lymphocyte populations.\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD\u2010DNA and RNA analysis was performed, under the same conditions, by capillary electrophoresis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared with the control group,\u00a0the\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD cohort presented significantly higher CD7, CD33 and CD123 levels. In order to assess the impact of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD abundance on antigen expression, the patients were grouped for each parameter into two cohorts using the following threshold values: (a) 0.5 for\u00a0the AR, according to current AML guidelines; (b) 0.7 for\u00a0the\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD\/\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010WT mRNA ratio (RR); and (c) 1.3 for the\u00a0\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD RR\/AR\u00a0ratio.\u00a0We found higher values of CD33 for RR\/AR\u00a0\u22651.3, and no other statistical differences between CD7, CD33 and CD123 levels of the other\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD groups. In terms of\u00a0correlations between MFI values and\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD parameters, we only observed a\u00a0moderate interdependence between CD33 MFI and the\u00a0RR\/AR ratio,\u00a0and\u00a0a weak negative correlation between CD123 MFI and AR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD mutations induce a specific antigen profile in AML blasts, and our data do not onfirm previous reports of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD AR influencing\u00a0both CD33 and CD123 expression.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcmm.15255","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T19:45:46Z","timestamp":1590608746000},"page":"7675-7679","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>FLT3<\/i>\n                    \u2010ITD DNA and mRNA levels in AML do not correlate with CD7, CD33 and CD123 expression"],"prefix":"10.1111","volume":"24","author":[{"given":"Dan\u2010Sebastian","family":"Soare","sequence":"first","affiliation":[{"name":"University Emergency Hospital Bucharest Bucharest Romania"},{"name":"Carol Davila University of Medicine and Pharmacy Bucharest Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5642-9979","authenticated-orcid":false,"given":"Eugen","family":"Radu","sequence":"additional","affiliation":[{"name":"University Emergency Hospital Bucharest Bucharest Romania"},{"name":"Carol Davila 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