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C57BL\/6J mice received third\u2010degree burns to 30% of the total body surface area. Rapamycin and 3\u2010methyladenine (3\u2010MA) were used to activate and inhibit autophagy, and the changes in LC3 and Beclin1 levels were assessed by Western blotting. The effect of autophagy on lncRNA H19 was detected by qRT\u2010PCR. Adenovirus\u2010mediated overexpression of lncRNA H19 in IEC\u20106 cells was used to assess the effects of lncRNA H19 on EGF and let\u20107g via bioinformatics analysis, Western blotting and qRT\u2010PCR. let\u20107g mimic\/inhibitor was used to overexpress\/inhibit let\u20107g, and qRT\u2010PCR and Western blotting were used to detect the effects of let\u20107g on EGF. The expression levels of LC3\u2010II, Beclin1 and lncRNA H19 were increased in intestinal tissues and IEC\u20106 cells after rapamycin treatment but were reversed after 3\u2010MA treatment. LC3\u2010II, Beclin1 and lncRNA H19 levels increased in intestinal tissues after the burn, and these increases were more significant after rapamycin treatment but decreased after 3\u2010MA treatment. The lncRNA H19 overexpression in IEC\u20106 cells resulted in increased and decreased expression levels of EGF and let\u20107g, respectively. Furthermore, overexpression and inhibition of let\u20107g resulted in decreased and increased expression of EGF, respectively. Taken together, intestinal autophagy is activated after a serious burn, which can increase the transcription level of lncRNA H19. lncRNA H19 may regulate the repair of EGF via let\u20107g following intestinal mucosa injury after a burn.<\/jats:p>","DOI":"10.1111\/jcmm.15262","type":"journal-article","created":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T03:40:56Z","timestamp":1587094856000},"page":"5878-5887","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Epidermal growth factor regulation by autophagy\u2010mediated lncRNA H19 in murine intestinal tract after severe burn"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9171-9437","authenticated-orcid":false,"given":"Cuijie","family":"Li","sequence":"first","affiliation":[{"name":"Department of Burn Surgery The Affiliated Huaihai Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Department of Burn Surgery The 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