{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:08:40Z","timestamp":1770329320082,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2015\/18\/E\/NZ9\/00607"],"award-info":[{"award-number":["2015\/18\/E\/NZ9\/00607"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chronic osteomyelitis, a bone infectious disease, is characterized by dysregulation of bone homeostasis, which results in excessive bone resorption. Lipopolysaccharide (LPS) which is a gram\u2010negative endotoxin was shown to inhibit osteoblast differentiation and to induce apoptosis and osteoclasts formation in vitro. While effective therapy against bacteria\u2010induced bone destruction is quite limited, the investigation of potential drugs that restore down\u2010regulated osteoblast function remains a major goal in the prevention of bone destruction in infective bone diseases. This investigation aimed to rescue LPS\u2010induced MC3T3\u2010E1 pre\u2010osteoblastic cell line using the methanolic extract of\n                    <jats:italic>Cladophora glomerata<\/jats:italic>\n                    enriched with\n                    <jats:italic>Mn(II)<\/jats:italic>\n                    ions by biosorption. LPS\u2010induced MC3T3\u2010E1 cultures supplemented with\n                    <jats:italic>C. glomerata<\/jats:italic>\n                    methanolic extract were tested for expression of the main genes and microRNAs involved in the osteogenesis pathway using RT\u2010PCR. Moreover, osteoclastogenesis of 4B12 cells was also investigated by tartrate\u2010resistant acid phosphatase (TRAP) assay. Treatment with algal extract significantly restored LPS\u2010suppressed bone mineralization and the mRNA expression levels of osteoblast\u2010specific genes such as runt\u2010related transcription factor 2 (\n                    <jats:italic>Runx2<\/jats:italic>\n                    ), alkaline phosphatase (\n                    <jats:italic>ALP<\/jats:italic>\n                    ) and osteocalcin (\n                    <jats:italic>OCN<\/jats:italic>\n                    ), osteopontin (\n                    <jats:italic>OPN<\/jats:italic>\n                    ),\n                    <jats:italic>miR\u201027a<\/jats:italic>\n                    and\n                    <jats:italic>miR\u201029b<\/jats:italic>\n                    . The extract also inhibited osteoblast apoptosis, significantly restored the down\u2010regulated expression of\n                    <jats:italic>Bcl\u20102<\/jats:italic>\n                    , and decreased the loss of MMP and reactive oxygen spices (ROS) production in MC3T3\u2010E1 cells induced by LPS. Furthermore, pre\u2010treatment with algal extract strongly decreased the activation of osteoclast in MC3T3\u2010E1\u20104B12 coculture system stimulated by LPS. Our findings suggest that\n                    <jats:italic>C. glomerata<\/jats:italic>\n                    enriched with\n                    <jats:italic>Mn(II)<\/jats:italic>\n                    ions may be a potential raw material for the development of drug for preventing abnormal bone loss induced by LPS in bacteria\u2010induced bone osteomyelitis.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15294","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T12:45:08Z","timestamp":1591274708000},"page":"7282-7300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<i>Cladophora glomerata<\/i>\n                    enriched by biosorption with\n                    <i>Mn(II)<\/i>\n                    ions alleviates lipopolysaccharide\u2010induced osteomyelitis\u2010like model in MC3T3\u2010E1, and 4B12 osteoclastogenesis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0660-8706","authenticated-orcid":false,"given":"Lynda","family":"Bourebaba","sequence":"first","affiliation":[{"name":"Department of Experimental Biology Faculty of Biology and Animal Science Wroc\u0142aw University of Environmental and Life Sciences Wroc\u0142aw Poland"},{"name":"International Institute of Translational Medicine Wisznia Ma\u0142a Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabela","family":"Michalak","sequence":"additional","affiliation":[{"name":"Department of Advanced Material Technologies Faculty of Chemistry Wroc\u0142aw University of Science and Technology Wroc\u0142aw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meriem","family":"Baouche","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology Faculty of Biology and Animal Science Wroc\u0142aw University of Environmental and Life Sciences Wroc\u0142aw Poland"},{"name":"International Institute of Translational Medicine Wisznia Ma\u0142a Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Kucharczyk","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology Faculty of Biology and Animal Science Wroc\u0142aw University of Environmental and Life Sciences Wroc\u0142aw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej M.","family":"Fal","sequence":"additional","affiliation":[{"name":"Collegium Medicum Institute of Medical Science Cardinal Stefan Wyszy\u0144ski University (UKSW) Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3676-796X","authenticated-orcid":false,"given":"Krzysztof","family":"Marycz","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology Faculty of Biology and Animal Science Wroc\u0142aw University of 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