{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:59:01Z","timestamp":1761238741726,"version":"3.40.5"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772874","81272965"],"award-info":[{"award-number":["81772874","81272965"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Long non\u2010coding RNAs (lncRNAs), which are longer than 200 nt, have been proved to play a role in promoting or inhibiting cancer progression. The following study investigated the role and underlying mechanisms of lncRNA RP11\u2010159K7.2 in laryngeal squamous cell carcinoma (LSCC) progression. Briefly, in situ hybridization (ISH) and real\u2010time quantitative PCR (RT\u2010qPCR) showed higher expression of RP11\u2010159K7.2 in LSCC tissues and cell lines. Patients with low expression level of RP11\u2010159K7.2 lived longer compared to those with high expression of RP11\u2010159K7.2 (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a039.111, ***<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Multivariate Cox regression analysis suggested that lncRNA RP11\u2010159K7.2 was an independent prognostic factor for LSCC patients (HR\u00a0=\u00a02.961, ***<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Furthermore, to investigate the potential involvement of RP11\u2010159K7.2 in the development of LSCC, we knocked out the expression of endogenous RP11\u2010159K7.2 in TU\u2010212 cells and AMC\u2010HN\u20108 cells via CRISPR\/Cas9 double vector lentiviral system. RP11\u2010159K7.2 knockout decreased LSCC cell growth and invasion both in vitro and in vivo. Mechanically, we found that RP11\u2010159K7.2 could positively regulate the expression of DNMT3A by sponging miR\u2010206. In addition, a feedback loop was also discovered between DNMT3A and miR\u2010206. To sum up, these findings suggest that lncRNA RP11\u2010159K7.2 could be used as a potential biomarker for prognosis and treatment of LSCC.<\/jats:p>","DOI":"10.1111\/jcmm.15331","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T07:56:06Z","timestamp":1588578966000},"page":"6781-6795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Regulation of laryngeal squamous cell cancer progression by the lncRNA RP11\u2010159K7.2\/miR\u2010206\/DNMT3A axis"],"prefix":"10.1111","volume":"24","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyu","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery Puyang Oilfield General Hospital Puyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Daqing Oilfield General Hospital Daqing China"},{"name":"Department of Otolaryngology Daqing First Hospital Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingrui","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin 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