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Long non\u2010coding RNA ANRIL (lncRNA\u2010ANRIL) has been reported to regulate endothelial functions in cardiovascular diseases. This study was to determine the role of lncRNA\u2010ANRIL in Akt regulation and cardiac functions after MI. Human umbilical vein endothelial cells (HUVECs) were exposed to oxygen\u2010glucose deprivation (OGD) to mimic in vivo ischaemia. The MI model in mice was induced by ligating left anterior descending coronary artery. OGD remarkably decreased lncRNA\u2010ANRIL expression level, reduced the phosphorylated levels of Akt and eNOS proteins, and inhibited NO release and cell viability, which were duplicated by shRNA\u2010mediated gene knockdown of lncRNA\u2010ANRIL. Conversely, all these effects induced by OGD were abolished by adenovirus\u2010mediated overexpression of lncRNA\u2010ANRIL in HUVECs. Further, OGD impaired cell migrations and tube formations in HUVECs, which were reversed by lncRNA\u2010ANRIL overexpression or Akt up\u2010regulation. RNA immunoprecipitation analysis indicated that the affinity of lncRNA\u2010ANRIL to Akt protein was increased in OGD\u2010treated cells. In animal studies, adenovirus\u2010mediated lncRNA\u2010ANRIL overexpression increased the phosphorylated levels of Akt and eNOS, promoted post\u2010ischaemic angiogenesis and improved heart functions in mice with MI surgery. LncRNA\u2010ANRIL regulates Akt phosphorylation to improve endothelial functions, which promotes angiogenesis and improves cardiac functions in mice following MI. In this perspective, targeting lncRNA\u2010ANRIL\/Akt may be considered to develop a drug to treat angiogenesis\u2010related diseases.<\/jats:p>","DOI":"10.1111\/jcmm.15343","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T08:40:26Z","timestamp":1589359226000},"page":"6860-6868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Overexpression of long non\u2010coding RNA ANRIL promotes post\u2010ischaemic angiogenesis and improves cardiac functions by targeting Akt"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-3134","authenticated-orcid":false,"given":"Qun","family":"Huang","sequence":"first","affiliation":[{"name":"Departmen of Pediatrics Xiangya Hospital Central South University Changsha China"},{"name":"Department of Child Health Care Hunan Provincial Maternal and Child Health Care Hospital Changsha 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