{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T11:07:00Z","timestamp":1793963220305,"version":"4.8.0"},"reference-count":32,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adipose\u2010derived stem cells (ASC) are said to have a pivotal role in wound healing. Specifically, ASC\u2010secreted extracellular vesicles (EV) carry diverse cargos such as microRNAs (miRNAs) to participate in the ASC\u2010based therapies. Considering its effects, we aimed to investigate the role of ASC\u2010EVs in the cutaneous wound healing accompanied with the study on the specific cargo\u2010medicated effects on wound healing. Two full\u2010thickness excisional skin wounds were created on mouse dorsum, and wound healing was recorded at the indicated time points followed by histological analysis and immunofluorescence staining for CD31 and \u03b1\u2010SMA. Human skin fibroblasts (HSFs) and human microvascular endothelial cells (HMECs) were co\u2010cultured with EVs isolated from ASC (ASC\u2010EVs), respectively, followed by the evaluation of their viability and mobility using CCK\u20108, scratch test and transwell migration assays. Matrigel\u2010based angiogenesis assays were performed to evaluate vessel\u2010like tube formation by HMECs in vitro. ASC\u2010EVs accelerated the healing of full\u2010thickness skin wounds, increased re\u2010epithelialization and reduced scar thickness whilst enhanced collagen synthesis and angiogenesis in murine models. However, miR\u2010486\u20105p antagomir abrogated the ASC\u2010EVs\u2010induced effects. Intriguingly, miR\u2010486\u20105p was found to be highly enriched in ASC\u2010EVs, exhibiting an increase in viability and mobility of HSFs and HMECs and enhanced the angiogenic activities of HMECs. Notably, we also demonstrated that ASC\u2010EVs\u2010secreted miR\u2010486\u20105p achieved the aforesaid effects through its target gene Sp5. Hence, our results suggest that miR\u2010486\u20105p released by ASC\u2010EVs could be a critical mediator to develop an ASC\u2010based therapeutic strategy for wound healing.<\/jats:p>","DOI":"10.1111\/jcmm.15387","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T00:44:00Z","timestamp":1594773840000},"page":"9590-9604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Extracellular vesicle\u2010enclosed miR\u2010486\u20105p mediates wound healing with adipose\u2010derived stem cells by promoting angiogenesis"],"prefix":"10.1111","volume":"24","author":[{"given":"Yingjie","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery The First Affiliated Hospital of Nanchang University Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huicai","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Plastic 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Nanchang University Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1096-0203","authenticated-orcid":false,"given":"Yuanlin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Burn Surgery The First Affiliated Hospital of Nanchang University Nanchang 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