{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:06:47Z","timestamp":1790816807519,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007300","name":"China Medical University","doi-asserted-by":"publisher","award":["DMR\u2010108\u2010035"],"award-info":[{"award-number":["DMR\u2010108\u2010035"]}],"id":[{"id":"10.13039\/501100007300","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005925","name":"Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMF\u2010SP 108\u201003"],"award-info":[{"award-number":["TCMF\u2010SP 108\u201003"]}],"id":[{"id":"10.13039\/501100005925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST\u2010108\u20102622\u2010B\u2010039\u2010002\u2010CC3"],"award-info":[{"award-number":["MOST\u2010108\u20102622\u2010B\u2010039\u2010002\u2010CC3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Functional decline of stem cell transplantation in ageing hosts is well documented. The mechanism for this is poorly understood, although it is known that advancing age does not provide an optimal milieu for exogenous stem cells to survive, engraft and differentiate. We showed that\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide improved human adipose\u2013derived stem cell (hADSC) engraftment via attenuating the production of reactive oxygen species (ROS). It remained unclear whether pre\u2010treated hosts with\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide can rejuvenate the ageing heart and improve hADSC engraftment by regulating the ROS\/NLRP3 inflammasome\u2010mediated cardiac fibrosis after myocardial infarction. One hour after coronary ligation, hADSCs were transplanted into the hearts of young and ageing Wistar rats that were pre\u2010treated with or without\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide for 3\u00a0days. At day 3 after infarction, myocardial infarction was associated with an increase in ROS levels and NLRP3 inflammasome activity with age. hADSC transplant effectively provided a significant decrease in ROS levels, NLRP3 inflammasome activity, IL\u20101\u03b2 levels and cardiac fibrosis in either young or old infarcted rats. However, the beneficial effects of hADSCs were greater in young compared with old rats in terms of NLRP3 inflammasome activity. The infarcted ageing rats pre\u2010conditioned by\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide improved engraftment and differentiation of hADSCs and additionally attenuated cardiac fibrosis compared with hADSCs alone. The anti\u2010inflammation effects of\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide were reversed by SIN\u20101. In conclusions, the increased NLRP3 inflammasome activity plays the pathogenesis of ageing\u2010related functional hADSC decline in the ageing hosts.\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butylidenephthalide\u2010pre\u2010treated ageing hosts reversibly ameliorate the harsh microenvironments, improve stem cell engraftment and attenuate cardiac fibrosis after myocardial infarction.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15403","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T17:52:59Z","timestamp":1602006779000},"page":"12272-12284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Host pre\u2010conditioning improves human adipose\u2013derived stem cell transplantation in ageing rats after myocardial infarction: Role of NLRP3 inflammasome"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3487-8749","authenticated-orcid":false,"given":"Tsung\u2010Ming","family":"Lee","sequence":"first","affiliation":[{"name":"Cardiovascular Institute An Nan Hospital China Medical University Tainan Taiwan"},{"name":"Department of Medicine China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horng\u2010Jyh","family":"Harn","sequence":"additional","affiliation":[{"name":"Bioinnovation Center Tzu Chi Foundation Hualien City Taiwan"},{"name":"Department of Pathology Buddhist Tzu Chi General Hospital Tzu Chi University Hualien City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzyy\u2010Wen","family":"Chiou","sequence":"additional","affiliation":[{"name":"Department of Life Science and Graduate Institute of Biotechnology National Dong Hwa University Hualien 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